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1、谷胱甘肽转硫酶M1基因型与胃癌易感性的研究作者：周涛樊薇韩炜高艳景袁孟彪李君曼李会庆李延青【摘要】目的：探讨中国汉族人群GSTM1基因型与胃癌易患性的关系，为胃癌高危人群的确定和胃癌的一级预防提供分子生物学手段。方法：501例，分为胃癌（GC）组、慢性萎缩性胃炎（CAG）组、慢性浅表性胃炎（CSG）组、胃溃疡（GU）组、十二指肠溃疡（DU）组及正常对照组，采用统一的调查表，调查年龄、性别、学历、职业、吸烟史、饮酒史、饮食习惯、肿瘤家族史等。采用序列特异性引物的聚合酶链反应（PCRSSP）检测GSTM1基因型测定组的基因型频率，ELISA法测
2、定Hp感染，病例对照研究方法进行流行病学分析。结果：单因素分析显示与胃癌有关联的危险因素为年龄、性别、文化程度、职业、HP感染、吸烟、GSTM1空白基因型，其中大蒜为保护性因素；多因素分析显示与胃癌有关联的危险因素分别为HP感染、GSTM1空白基因型，大蒜仍为保护性因素。HP感染与GSTM1空白基因型对胃癌的发生存在协同作用；吸烟与GSTM1空白基因型存在协同作用。结论：具有GSTM1基因型的个体发生胃癌的危险性增加，该基因型可以认为是胃癌易患性的标志。检测该基因型能够对胃癌高危人群的确定，对胃癌的早期发现和一级预防提供有益的线索。【关键词
3、】胃肿瘤・基因型・流行病学，分子ThestudyoftheassociationofGSTM1genepolymorphismwithsusceptibilitytogastriccancer【ABSTRACT】Objective:ToexploretheassociationofGSTM1genepolymorphismwithsusceptibilitytogastriccancerinChineseHannationalityinordertoprovidemolecularmeasuretodefinet
4、hehighriskpopulationofgastriccancerandforprimaryprevention.Methods:AllsubjectshavebeendividedintoGastriccancer(GC),chronicatrophicgastritis(CAG),chronicsuperficialgastritis(CSG),gastriculcer,duodenalulcerandcontrolgroupswhichhadbeendiagnosedbymeansofendoscopicalandhistopat
5、hologicalobservation.Dataonlifestylehavebeenroutinelycollectedbymeansofcommonquestionnairesheets.Thequestionnairecomprisesquestionsonage,sex,education,occupation,smokinganddrinking,dietaryhabitsandfamilyhistoryofcancer,etal.GSTM1genotypesweredetectedbypolymerasechainreacti
6、onofsequencespecificprimers(PCRSSP).Hpinfectionwasdetectedbyenzymelinkedimmunosorbentassay(ELISA).Theapproachofcasecontrolwasusedbytheanalysesofriskfators.Results:Theriskfactorsassociatedwiththedevelopmentofgastriccancerwereage,sex,education,occupation,helicobacterpylori5i
7、nfection,tobaccosmoke,GSTM1nullgenotype,garlicwasprotectivefactor.Multivariateanalysishaveshownthattheriskfactorswerehelicobacterpyloriinfection,GSTM1nullgenotype,garlicwasalsoaprotectivefactor.ThereissynergisticactionbetweenhelicobacterpyloriinfectionandGSTM1genotype,toba
8、ccosmokeandGSTM1genotypeinthedevelopmentofgastriccancer.Conclusion:TheGSTM1nullgenotype,h
