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1、OnbreastcancerinERPRandHER2expressionanditssignificance[Abstract]ObjectiveToinvestigateHER2,ER,PRproteinregioninthenorth,theexpressionofbreastcancerpatients,andHER2andER,PRexpressionbetweenthecorrelationmethodofthenorth,areasofbreastcancersurgicalspecimenswere10%neutralformalin-fixed,two-stepmeth
2、odusingSPcombinedimmunestainingresultsofHER2proteinexpressionwasnegativelycorrelatedwithERandPRexpressionalsoshowedanegativecorrelation.[Keywords]ERPRHER2breastcancerBreastcancerisharmfultowomen’shealthfirst,theincidencerateshowedagradualupwardtrend.Breastcancerisasystemicdisease,theexactcausehas
3、notyetfullyunderstood.Tumorinvasionandmetastasisisamulti-generegulation,multi-step,multi-stageofevolution,whichistheabnormalproliferationoftumorcellsintumorinvasionandmetastasisbasedonchangesinadhesionandenhancedexercisecapacity,andlossof12contactinhibitionistheextracellularconditionsofinvasionan
4、dmetastasisinbreastcancer-relatedproteinsassociatedwithprognosisandtreatmentmainlyforER,PRandHER2,understandingtheexpressionofthesethreeproteinsisthekeytobreastcancertreatment.1Materialsandmethods1.1ResearchmaterialcollectedinJanuary2006-December2009People’sHospitalduringtheBeiliupathologicaldiag
5、nosisofbreastcancersurgicalspecimensof84cases,37-year-oldminimumage,maximum71years,meanage51years,medianage53yearsAllspecimenswere10%neutralformalin-fixed,paraffin-embeddedsections,allspecimenswerere-productionandre-readthepieceinordertofurtherclarifythediagnosis.1.2ResearchMethodsAllimmunohistoc
6、hemistryreagentswerepurchasedfromFujianstepthenewcompany,readytouse.Two-stepmethodusingSPimmunohistochemicalstaining,concentratedDABchromogenickit,stepsinaccordancewithinstructions.121.3DetectionAfterallspecimenswerefixedin10%neutralformalin,conventionaldehydrated,embeddedinparaffin,sliced??thick
7、nessof4μm,usingconventionalimmunohistochemistrySPstainingmethodspecificmethodisasfollows:AllslideswereconfirmedbyAPES-treatedfilm,4μmserialsections,dewaxingtohydration,3%hydrogenperoxideatroomtemperaturefor10minutestob
