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时间:2018-08-08
《鸡源大肠埃希菌的耐药性与分子分型研究》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、鸡源大肠埃希菌的耐药性与分子分型研究钱晓璐1,商军1*,董栋2,张浩然1,田恺1(1.上海市兽药饲料检测所,上海,201103;2.复旦大学附属华山医院,上海,200040)[收稿日期][文献标识码][文章编号][中图分类号][摘要]为了解目前规模化养殖厂鸡源大肠埃希菌的耐药性以及分子流行规律和特征,为该菌的分子溯源及风险评估提供依据,本研究收集2013~2014年的鸡源大肠埃希菌共计741株,采用微量稀释肉汤法分别对常用的9类13种抗菌药物进行药物敏感性测定,以了解大肠埃希菌的耐药表型。在筛选出多重耐药严重的菌株后,同时采用脉冲场凝
2、胶电泳(PFGE)分型方法及核糖体(RP)分型方法进行分子分型分析。结果显示,2013~2014年所分离的鸡源大肠埃希菌对氨苄西林、阿莫西林/克拉维酸、大观霉素、四环素、多西环素、氟苯尼考、磺胺异噁唑、复方新诺明、氧氟沙星和恩诺沙星的耐药率较高,在50%以上,且主要为多重耐药菌株。用XbaⅠ酶切后进行PFGE分析,结果表明,29株鸡源大肠埃希菌可分为22个带型;用EcoRⅠ酶切之后,RP聚类分析结果表明,鸡源大肠埃希菌分为14个带型。鸡源大肠埃希菌耐药率较高,表现出较为严重的耐药性,同一分型菌株的耐药谱型并非完全一致,菌株基因型呈多态
3、性。[关键词]大肠埃希菌;耐药性;PFGE;RP分型;分子分型StudyonAntimicrobialResistanceandMolecularSubtypingofEscherichiacoliIsolatedfromChickensQIANXiao-lu1,SHANGJun1*,DONGDong2,ZHANGHao-ran1,TIANKai1(1.ShanghaiInstituteforVeterinaryDrug&Feedscontrol,Shanghai,201103;2.HuashanHospitalAffiliatedt
4、oFudanUniversity,Shanghai,200040;China)Abstract:ThisstudywasconductedtoinvestigatetheantimicrobialresistanceandobtainthesubtypingofEscherichiacoliisolatedfromchickens.741strainsofEscherichiacoliwerecollectedfromdifferentareasinShanghaiintwoyears(2013-2014).Susceptibilit
5、ytestto13antimicrobialagentswasperformedbybrothmicrodilution.Pulsed-fieldgelelectrophresis(PFGE)andRibo-printer(RP)wereusedtosubtypethemultidrugresistantstrains.AccordingtotheantimicrobialsusceptibilitytestofEscherichiacoli,theresistanceratestoampicillin,amoxicillin/cla
6、vulanicacid,spectinomycin,tetracycline,doxycycline,florfenicol,sulfonamideisoxazole,trimenthoprim-sulfamethoxazole,ofloxacinandenrofloxacinweregenerallyhigh..Andmorethan80%multidrugresistantstrainsweredetected.ResultsofPFGEshowedthatthesestrainsofEscherichiacoliweredivi
7、dedinto22typesafterdigestedbyXbaⅠ.WhileresultsofRPdividedthesestrainsinto14typesafterdigestedbyEcoRⅠ.ComparedwithRP,PFGEhadhigherdefinitiononmolecularsubtyping.ThisstudyshowedthatthegenetypeofE.coliwaspolymorphic.Andphenotypesofisolateswiththesamesubtypingwerenotexactly
8、identical.Keywords:Escherichiacoli;antimicrobialresistance;PFGE;Ribotyping;molecularsubtyping大肠埃希菌(Escherichia
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