sle病人及其子代与sle相关基因及抗体关系研究

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1、sle病人及其子代与sle相关基因及抗体关系研究sle病人及其子代与sle相关基因及抗体关系研究本文来源：论文http://www.xzbu.com/作者：孙焕霞苏厚恒崔佳佳李光文【摘要】目的探讨缓解期系统性红斑狼疮(sle)病人所生子女罹患sle的危险性。方法应用实时定量pcr技术检测14例sle病人、病人子女及18例对照者hla?drb1*1501、drb1*0301、dqa1*0102、dqb1*0602的频率，免疫印迹法检测入组者ena抗体谱。结果sle病人组dqa1*0102频率显著高于对照组(rr=2.02,p<0.05)，而两组间drb1*1501、drb1*

2、0301、dqb1*0602频率比较差异无显著意义(p>0.05);sle病人组dqb1*0602频率显著高于其子女组(rr=2.06,p<0.05)，而两组间drb1*1501、drb1*0301、dqa1*0102频率比较差异无显著性(p>0.05);sle子女组dqa1*0102频率显著高于对照组(rr=2.14，p<0.05);而两组间drb1*1501、drb1*0301、dqb1*0602频率比较差异无显著性(p>0.05)。sle病人组自身抗体ssa及dsdna出现频率明显高于对照组(p=0.019、0.015)，两组间ssb及ro?5

3、2差异无显著性(p>0.05);sle病人子女组所测自身抗体检出率为0。结论hla?dqa1*0102为sle的易感等位基因，dqa1*0102有一定的遗传倾向。经过系统治疗达到缓解期的sle病人生育的子女罹患sle的危险性无明显增加趋势。【关键词】红斑狼疮,系统性;hla抗原;疾病遗传易感性;等位基因abstractobjectivetoexploretheriskofsufferingfromsystemiclupuserythematosus(sle)inchildrenbornbypatientswithsleatparacmasis.methodsthefrequ

4、enciesofhla?drb1*1501,drb1*0301,dqa1*0102anddqb1*0602allelesof14slepatientsandtheirchildren,and20healthycontrolsweredetectedbyreal?timepcrtechnologyandenaantibodyrepertoirebyimmunoblotting.resultsinslegroup,thefrequencyofdqa1*0102allelewassignificantlyhigherthanthatinthecontrols(rr=2.02,p<

5、0.05),andnosignificantdifferencesoffrequenciesofdrb1*1501,drb1*0301,anddqb1*0602allelewerenotedbetweenthetwogroups(p>0.05);thefrequencyofdqb1*0602alleleinslepatientswassignificantlyhigherthanthatoftheirchildren(rr=2.06,p<0.05),andnosignificantdifferencewasfoundbetweenthemintermsofdrb1*1

6、501,drb1*0301,dqa1*0102allele(p>0.05).insle?childrengroup,dqa1*0102allelefrequencywassignificantlyhigherthanthatofthehealthycontrols(rr=2.14,p<0.05),andthedifferencesofdrb1*1501,drb1*0301,anddqb1*0602allelewerenotsignificantbetweenthetwogroups.thefrequenciesofautoantibodiesssaanddsdnain

7、slepatientswerehigherthanthatofthecontrols(p=0.019,0.015),andnostatisticaldifferencesofssbandro?52wererecordedbetweenthetwogroups(p>0.05).thedetectionrateofautoantibodywasnilinthepatients’children.conclusionhla?dqa1*0102isasusceptibleallel