原花青素对紫外线诱导晶状体上皮细胞氧化损伤保护作用的研究

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1、原花青素对紫外线诱导晶状体上皮细胞氧化损伤保护作用的研究【摘要】目的：研究原花青素(procyanidins,PC)对紫外线诱导的人晶状体上皮细胞(lenepithelialcells,LECs)DNA氧化损伤的保护作用。方法：采用照射剂量为15mJ/cm2的中波紫外线(UVB)分别照射用0(对照组)，0.05，0.1，0.2mg/mL的PC，牛磺酸(TAU)，维生素C(VC)预处理的LECs，未处理组未给予紫外线和任何抗氧化剂处理，用单细胞凝胶电泳法(SCGE)检测分析各PC组LECsDNA单链断裂的程度，并与其他各

2、组的检测结果相比较。结果：各PC组的尾长、尾部DNA百分比、尾力矩在数值上均明显小于对照组，且与对照组相比，差异均有统计学意义(P<0.01)。各PC实验组的尾部DNA百分比、尾力矩与未处理组相比差异均无统计学意义(P>0.05)。相同浓度下，PC，TAU和VC组的各检测指标数值逐渐增大。结论：外源性PC对UVB照射诱导损伤的人LECsDNA有保护作用。PC的保护作用明显强于TAU和VC，并且在一定浓度范围内随着浓度的增加其抗氧化作用逐渐增强。【关键词】原花青素;紫外线;晶状体上皮细胞;DNA损伤　　Abs

3、tractAIM:Todeterminetheprotectionofprocyanidins(PC)againstUVBinducedoxidativedamageoflensepithelialcells(LECs)withalkalinesinglecellgelelectrophoresis,inordertoprovideanexperimentalfoundation13forcataractclinicaltreatment.METHODS:LECslinesofgeneration4weredivid

4、edrandomlyinto5groups.Asuntreatedgroup:normalculturedLECs;controlgroup:LECs+UVB;threetreatedgroups:PCtreatedgroups:LECs+PC+UVB;taurine(TAU)treatedgroups:LECs+TAU+UVB;VCtreatedgroups:LECs+VC+UVB.Allgroupsofantioxidantsweredividedinto3groupsaccordingtothefinalco

5、ncentrationfromlowtohigh(0.05,0.1,0.2mg/mL).Eachtreatedgroupwastreatedbyantioxidants2hoursbeforeirradiatedunderUVB,respectively,thenconventionalcultivation.TheLECsoftreatedgroupsandcontrolgroupwereallirradiatedatUVBdose15mJ/cm2.AllofLECsineverygroupwerecollecte

6、daftertreatmentanhourrespectivelyandanalyzedwithalkalinesinglecellgelelectrophoresis.RESULTS:Thedifferencesoftaillength,percentageoftailDNAandtailmomentbetweenPCtreatedgroupsandcontrolgroupwerestatisticallysignificant(P<0.01).ThedifferencesofpercentageoftailD

7、NA,tailmomentineachPCtreatedgroupanduntreatedgroupwerenotsignificant(P>0.05).TheresultsofPCtreatedgroupsweresignificantlysmallerthanthoseofTAUtreatedgroupsandVCtreatedgroups,Thedifferencesoftaillength,percentageoftailDNAandtailmomentinvariousantioxidanttrea

8、tedgroupswere13statisticallysignificant(P<0.01).CONCULSION:ExogeneticPChasprotectiveeffecttoUVBinducedLECsDNAdamage.TheprotectiveeffectofPCisobviouslystrongerthan