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1、凉血活血法对肺纤维化小鼠羟脯氨酸和转化生长因子-β1含量的影响作者：胡静,郭永洁,刘旭凌,符胜光,庞慧芳【摘要】目的研究凉血活血法防治肺纤维化的作用,并探讨其作用机制。方法将雄性ICR小鼠随机分为4组：阴性对照组、肺纤维化模型组、凉血活血法组、强的松阳性对照组。经鼻滴入博莱霉素建立小鼠肺纤维化模型,造模8h开始常规灌胃给药。应用凉血活血法(犀角地黄汤)防治28d后进行取材及实验指标检测：用酶联免疫吸附法测定支气管肺泡灌洗液(BALF)中转化生长因子-β1(TGF-β1)含量变化,用样本碱水解法检测肺组织中羟脯氨酸(HYP)的含量。结果肺组织病理形态
2、学观察发现模型组小鼠肺泡炎及纤维化程度明显高于阴性对照组；与模型组比较,凉血活血法组肺组织中HYP含量显著降低(P＜0.01),BALF中TGF-β1含量显著降低(P＜0.01)。结论凉血活血法能明显减轻博莱霉素所致的小鼠肺纤维化程度,降低肺组织中HYP含量。其机制可能是通过抑制TGF-β1蛋白表达,减少胶原蛋白的产生,使肺纤维化病变减轻。【关键词】肺纤维化；转化生长因子-β1；羟脯氨酸；凉血活血法；小鼠Abstract：ObjectivesTostudytheeffectofcoolingblood10andinvigoratingcircula
3、tionmethodonpreventionandtreatmentofpulmonaryfibrosis,andexploreitsmechanism.MethodsMaleICRmicewererandomlydividedinto4groups：negativecontrolgroup,pulmonaryfibrosismodelgroup,coolingbloodandinvigoratingcirculationgroupandprednisonepositivecontrolgroup.Thepulmonaryfibrosismodel
4、ofmicewereestablishedbyBLMnasalinstillation.After8hours,themiceweregivenprescriptionofcoolingbloodandinvigoratingcirculation(XijiaoDihuangdecoction)bygastricperfusion.Atthe28thdayaftertreatment,theindexesweredetected.TheTGF-β1contentofbronchoalveolarlavagefluid(BALF)wastestedb
5、yenzymelinkedimmunosorbentassay,andhydroxyproline(HYP)wasexaminedbyalkalinehydrolysis.ResultsThedegreeofairsacculitisandfibrosisinmodelgroupareobviouslyhigherthanthenegativecontrolgroupbypathologicalobservation.Comparedwithmodelgroup,HYPcontentwasdecreasedobviouslyinherbgroup(
6、P＜0.05).Comparedwithmodelgroup,TGF-β1contentwasincreasedobviouslyinherbgroup(P＜0.05).ConclusionsThemethodofcoolingbloodandinvigoratingcirculationcanreducethedegreeofairsacculitisandfibrosis,anddecreasethecontentofHYPandTGF-β1inlungtissueandBALF.Itspossiblemechanismmayberelatet
7、oinhibitingTGF-β101expressionanddecreasingcollagenproteinproduction,whichleadtothereductionofdegreeofairsacculitisandfibrosis.Keywords：pulmonaryfibrosis；TGF-β1；hydroxyproline；coolingbloodandinvigoratingcirculationmethod；mice2003年,传染性非典型肺炎肆虐,在其病理演变过程中可出现不同程度的纤维化病理改变[1]。西医以皮质激素和
8、细胞毒药物治疗,但仅有10%～30%肺纤维化患者经糖皮质激素治疗有一定的疗效[2],且激素不良反应大,常出现大面积股骨头坏
