川芎嗪对大鼠局部脑缺血后空间学习和记忆的影响

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1、川芎嗪对大鼠局部脑缺血后空间学习和记忆的影响作者：赵建军，刘勇，陈新林，刘建新，田英芳，邱芬，张蓬勃，康前雁【关键词】川芎嗪　　摘要：目的探讨川芎嗪对大鼠局部脑缺血后空间学习记忆能力障碍的作用。方法线栓法制作大鼠大脑中动脉阻塞(MCAO)模型，术后2周内腹腔注射川芎嗪，第15天开始采用Morris水迷宫装置评价大鼠的空间学习记忆能力，脑切片尼氏染色观察皮质和海马的神经元数量变化。结果脑缺血对照组大鼠在定向航行试验和空间探索试验中均表现出明显的空间认知功能的障碍，川芎嗪治疗组大鼠平均逃避潜伏期与缺血对照组比较明显缩短(P<0.01)。在空间探索试验中，川芎嗪治疗组大鼠原平台

2、象限停留时间百分比以及穿过原平台位置次数均大于脑缺血对照组(P<0.01)。在形态学观察中，川芎嗪治疗组大鼠缺血侧顶叶皮质神经元数量明显多于脑缺血对照组(P<0.01)，各组动物海马神经元数量上无统计学差异。结论川芎嗪可以明显改善大鼠永久性局部脑缺血后空间学习记忆能力，其机制可能与川芎嗪对神经元的保护作用有关。　　关键词：川芎嗪；学习记忆；morris水迷宫；脑缺血12　　ABSTRACT:ObjectiveToinvestigatetheeffectsoftetramethylpyrazineonfunctionofspatiallearningandmemory

3、inratafterfocalcerebralischemia.MethodsThefocalcerebralischemicmodelwasestablishedbyintroducinganintraluminalfilamentintotheleftmiddlecerebralarteryoftherat,thentetramethylpyrazinewasadministeredintroperitoneallyaftertheoperationandafterwardonceadayfor2weeks.Thespatiallearningandmemoryfuncti

4、onwasstudiedbyMorriswatermazetest.Nisslstainingtechniquewasconductedforobservingthemorphologyofneuronsincortexandhippocampus.ResultsTheischemiacontrolgroupexhibitedseriouslyspatiallearningandmemorydeficitsinbothplacenavigationtrailandspatialprobetrial.Inplacenavigationtrial,themeanvaluesofes

5、capelatencyoflowerdose,middledoseandhigherdosetetramethylpyrazinegroupswereshorterthanthoseofischemiacontrolgroup(P<0.01).Inspatialprobetrial,thereweresignificantdifferenceinthetimepercentageofspendingintheformerplatformquadrantandfrequencyofcrossingtheformerplatformsitebetweenlower,middl

6、eandhigherdosetetramethylpyrazinegroupsandischemiacontrolgroup(P<0.01).Comparedwithischemiacontrolgroup,lower,12middleandhigherdosetetramethylpyrazinegroupshadmoreneuronsofnisslstaininginipsilateralparietalcortex(P<0.01).Thecellnumbersoftotalneuronsofhippocampushadnosignificantdifferen

7、cebetweenlower,middleandhigherdosetetramethylpyrazinegroupsandischemiacontrolgroup.ConclusionTetramethylpyrazineimprovestheratspatiallearningandmemoryabilityafterfocalcerebralischemia,andtheneuroprotectiveroleofthedruginnewcortexmaybeinvolvedinitsm