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1、姜黄素与依那普利对单侧输尿管梗阻大鼠模型干预的对比研究作者：赵爱青,张晓明,侯恒,李荣山,马存根【摘要】目的观察姜黄素对单侧输尿管梗阻(UUO)大鼠肾间质纤维化的影响,并与依那普利进行比较。方法采用UUO致肾间质纤维化大鼠模型,术后第4周处死各组大鼠,用HE、Masson染色评定肾小管间质纤维化程度；用免疫组化方法检测肾组织内转化生长因子β1(TGF-β1)、结缔组织生长因子(CTGF)和基质金属蛋白酶抑制剂－1(TIMP-1)的表达部位及蛋白表达水平。结果与假手术组相比,模型组大鼠肾间质纤维组织面积显著
2、扩大(P<0.01),肾组织内TGF-β1、CTGF和TIMP-1蛋白质表达均显著上调(P<0.01)。姜黄素或依那普利干预后,上述上调指标都被显著抑制(P<0.05或P<0.01)。两种药物作用比较无显著差异(P>0.05)。结论姜黄素或依那普利对UUO大鼠的干预效果相似,此可能与下调肾组织内TGF-β1和CTGF,进而部分抑制TIMP-1的表达有关。【关键词】姜黄素；依那普利；转化生长因子β1；结缔组织生长因子；基质金属蛋白酶抑制剂-1；肾间质纤维化 Abstract：
3、ObjectiveToinvestigatetheeffectofcurcuminonrenalinterstitialfibrosisfollowingunilateral10ureteralobstruction(UUO),andcomparetheeffectsofCurcuminwiththatofEnalapril.MethodUUOratmodelwasused.MaleWistarratswererandomlydividedintofourgroupswith8ratseach：shame
4、-operatedgroup(0.9%salinesolution),modelgroup(dimethylsulphoxide,DMSO),Curcumintreatmentgroup[curcumin100mg/(kg·d)],andEnalapriltreatmentgroup[enalapril10mg/(kg·d)].Allratswerekilled4waftersurgery.ThedegreeoftubulointerstitialfibrosiswasscoredbyHEandMasso
5、nstaining.ThesitesandlevelsofproteinexpressionofTGF-β1,CTGFandTIMP-1weredetectedbyimmunohistochemistrystaining.ResultComparedwiththoseofshame-operatedgroup,thebloodureanitrogenlevelwassignificantlyincreased(P<0.01),andtherelativeareaofinterstitialfibro
6、siswasalsosignificantlyenlarged,theexpressionofTGF-β1,CTGFandTIMP-1proteinwassignificantlyup-regulatedinkidneytissueofmodelgroup(P<0.01).AfterinterventionwithCurcuminorEnalapril,theupregulationoftheabove-mentionedparameterswereallsignificantlyinhibited
7、(P<0.05orP<0.01).TherewasnosignificantdifferenceofinterventioneffectsbetweenCurcuminandEnalapril(P>0.05).ConclusionPharmacologicaleffectsofCurcuminorEnalaprilonunilateralureteralobstructionratsarequitesimilar.Theseeffectsmay10resultfromthedown-re
8、gulationofTGF-β1,CTGFandTIMP-1expressioninkidneytissue. Keywords：Curcumin；Enalapril；TGF-β1；CTGF；TIMP-1；renalinterstitialfibrosis 姜黄素(Curcumin)是从姜科植物姜黄中提取的一种植物多酚,也是姜黄发挥药理作用最重要的活性成分。近年研究表明,姜黄素对多种原因所致的肾损害、糖尿病大鼠肾脏都具有保
