大鼠急性脑缺血再灌注后血脑屏障通透性的超微结构与血清s100b蛋白水平的动态变化

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1、大鼠急性脑缺血再灌注后血脑屏障通透性的超微结构与血清S100B蛋白水平的动态变化作者：吴松笛，万琪，夏峰，耿晓英，刘学东，王津存【关键词】血脑屏障　　DynamicchangesofbloodbrainbarrierpermeabilityandS100Bproteinlevelinserumafteracutecerebralischemia/reperfusioninrats　　【Abstract】AIM:Toinvestigatethedynamicchangesofthebloodbrainbarrier（B

2、BB）permeabilityandtheS100Bproteinlevelinserumafteracutecerebralischemia/reperfusioninrats.METHODS:Themiddlecerebralarteryocclusion/reperfusion(MCAO/R)ratmodelwasmadebythethreadocclusionmethod;thepermeabilityofBBBwasinvestigatedbytransmissionelectronmicroscopeus

3、inglanthanumnitrateasthetracer;theserumS100BproteinconcentrationsweredeterminedbyELISAat0.5,2,6,12,24and48hreperfusionafter2hcerebralischemia.RESULTS:①Electronmicroscopyrevealedthatlanthanumgranulesappearedaroundthetightjunctionandintheendotheliocytesat0.5h11ar

4、eperfusion,indicatingtheincreaseofBBBpermeability.Morephagotrophiclanthanumgranuleswerefoundintheendotheliocytesthatshowedsignificantvacuolizationat2and6h.Thetightjunctionwasopenedandtheastrocyteswereseparatedfromthebasementmembraneofthecapillaries.Thenecrosiso

5、fbothneuronsandastrocyteswithdissolvednucleusandthedisruptionofthecapillarybasementmembranewerefoundat12and24h.Thenecrosisanddisaggregationofmanyneuronsandastrocyteswereevidentat48h.Alotoflanthanumgranuleswereshownoutsidethebrainvesselswhilemanyendotheliocytesw

6、erebroken.②TheserumS100Bproteinlevelincreasedsignificantlyonlyinratsaftercerebralischemia/reperfusioninjury,butnotintheshamoperationandthenonoperationcontrolrats(P<0.01).TheserumconcentrationofS100Bproteinstartedtoincreaseat0.5hofreperfusion.Itkeptatahighlev

7、eluntilapeakappearedat24h.TheserumS100Bproteinwasmaintainedatahighlevelconstitutivelyevenat48h.CONCLUSION:ThedynamicchangeoftheS100Bproteinlevelinserumafterischemia/reperfusionreflectsthechangeofthepermeabilityofBBB.S100Bcanthereforebeusedasaperipheralbiochemic

8、almarkertoassessthepermeabilityofBBB.11　　【Keywords】bloodbrainbarrier;brainischemia;reperfusion;S100Bprotein　　【摘要】目的：研究大鼠急性脑缺血再灌注后血脑屏障通透性的超微结构与血清S100B蛋白水平的动态变化.方法：采用线栓法制作大鼠大脑