l-精氨酸对糖尿病大鼠坐骨神经损伤的保护作用

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1、L-精氨酸对糖尿病大鼠坐骨神经损伤的保护作用作者：陈秀芳，董敏，金晓冬，雷康福金丽琴【摘要】目的:探讨L-精氨酸（L-Arg）对糖尿病(DM)大鼠坐骨神经损伤的保护作用及其机制。方法:用链脲佐菌素制备糖尿病模型。SD大鼠随机分为正常对照组（NC组）、DM组、L-Arg治疗组（L-Arg组）。L-Arg治疗12周后，测定三组大鼠血清、坐骨神经组织一氧化氮(NO)含量和一氧化氮合酶(NOS)活性及血浆内皮素-1(ET-1)、降钙素基因相关肽(CGRP)含量，以及坐骨神经组织Ca2+-ATPase、N

2、a+-K+-ATPase活性及神经元型NOS（nNOS）、醛糖还原酶（AR）基因的表达。结果:DM组大鼠血清、坐骨神经NO含量和NOS活性、血浆CGRP含量及坐骨神经Ca2+-ATPase、Na+-K+-ATPase活性和nNOSmRNA的表达均明显低于NC组（P<0.01或P<0.05），而血浆ET-1含量、坐骨神经ARmRNA的表达均明显高于NC组（均P<0.01）；经L-Arg治疗后，上述改变逆转，与DM组比较差异有显著性（P<0.01或P<0.05）。结论:

3、L-Arg对DM大鼠坐骨神经损伤具有一定的保护作用，其机制可能与改善神经血供及抑制多元醇代谢途径有关。【关键词】L-精氨酸；糖尿病；坐骨神经；一氧化氮；内皮素-1；降钙素基因相关肽；醛糖还原酶；大鼠11Abstract:Objective:ToinvestigatetheeffectsandmechanismsofL-arginine(L-Arg)onpreventingandtreatingsciaticnerveinjuryofexperimentaldiabeticrats.Methods

4、:Thediabetesmellitus(DM)ratsmodelsweremadebyintraperitonealinjectionofstreptozotocin(60mg/kgbodyweight),andthreegroups,includingnormalcontrolgroup（NCgroup）,DMgroup,L-Argtreatmentgroup(L-Arggroup)wereallocatedatrandom.AfterthesupplementationofL-Arg(30

5、0mg·kg-1·d-1)for12weeks,theratsweresacrificed.Thenitricoxide(NO)contentandnitricoxidesynthase(NOS)activityinserumandsciaticnerve,plasmaendothelin-1(ET-1)andcalcitoningene-relatedpeptide(CGRP)contentweredetermined.TheactivitiesofCa2+-ATPase,Na+-K+-ATP

6、aseandthemRNAexpressionlevelofneuronalNOS(nNOS)andaldosereductase(AR)ofsciaticnervealsoweremeasured.Results:TheNOcontentandNOSactivityinserumandsciaticnerve,plasmaCGRPcontent,andCa2+-ATPase,Na+-K+-ATPaseactivitiesandnNOSmRNAexpressionlevelinsciaticne

7、rveofDMgroupweresignificantlylowerthanthoseofNCgroup(P<0.01orP<0.05respectively),butplasmaET-1contentandARmRNAexpressionlevelinsciaticnerveweresignificantlyhigherthanthoseof11NCgroup(P<0.01respectively).ButinL-Arggroup,theabovechangeswererev

8、ersed,thereweresignificantdifferencescomparedwiththoseoftheDMgroup(P<0.01orP<0.05respectively).Conclusion:L-Argplayspreventiveandremedialrolesindiabeticsciaticnerveinjury.Improvingthelevelbloodsupplyingandinhibitingpolyolmetabolicpathwaymaybeit