低强度脉冲超声波影响兔骨髓基质干细胞与β-磷酸三钙复合修复骨缺损的实验研究

《低强度脉冲超声波影响兔骨髓基质干细胞与β-磷酸三钙复合修复骨缺损的实验研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、低强度脉冲超声波影响兔骨髓基质干细胞与β-磷酸三钙复合修复骨缺损的实验研究作者：梁伟，苟三怀，欧阳跃平，刘岩，严力生，陈洪勋【摘要】［目的］探讨低强度脉冲超声波(LIPUS)影响β-磷酸三钙(β-TCP)与兔骨髓基质干细胞(BMSCs)复合体修复兔桡骨骨缺损的效果。［方法］从兔股骨粗隆部抽取骨髓，体外培养扩增，取第3代BMSCs接种于β-TCP培养1周，分为两组，一组予以LIPUS作用，另一组作为对照。以20只成年新西兰兔为研究对象，制作双侧桡骨远端骨缺损模型。一侧桡骨骨缺损处植入LIPUS作用过的细胞支架

2、复合体做为实验组，另一侧植入LIPUS未作用过的细胞支架复合体做为对照组。分别于术后4周、8周处死新西兰兔，运用计算机图像分析X线片上骨痂的灰度密度，行组织学切片检查骨痂生长情况。［结果］X线片骨痂灰度密度测量提示实验组骨痂较对照组生长明显增多。组织学切片显示实验组血肿机化、吸收、骨小梁和骨基质形成早于对照组。［结论］在骨缺损修复早期，LIPUS对BMSCs与β-TCP复合体修复兔骨缺损有明显的促进作用；而在骨缺损修复晚期，LIPUS的作用相应减弱。【关键词】骨组织工程；细胞支架复合体；骨缺损；低强度脉冲超

3、声波13Abstract:［Objective］Toexploretheeffectoflowintensitypulsedultrasound(LIPUS)ontheabilityofrepairingrabbitradiusdefectbycell-scaffoldcomplexcomposedwithbonemarrowstromalcells(BMCs)andβ-tricalciumphosphate(β-TCP).［Method］Bonemarrowofarabbitwasdrawnandcult

4、uredbydifferentiationculturemediumtoharvestBMCsandthethirdgenerationBMCswerecultivatedonβ-TCPforoneweekinvitro.Bilateraldistalradialdefectsweremadeusinga1-mmsawin20matureNewZealandwhiterabbits.Thecell-scaffoldcomplexsstimulatedbyLIPUSwereimplantedinrabbitr

5、adialdefects(cell-scaffoldcomplexswithoutstimulationascontrol).Alltherabbitswerekilledat4or8weeksafteroperation.ThedefecthealingstatewasobservedwithX-rayandhistologicaltechnic.ThegraydensityofCAwasanalyzedbycomputerimageanalysissystem.［Result］Itwasfoundtha

6、tthecallusintheexperimentalgroupgrewmorequicklyandapparentlythanthatinthecontrolgroup.At4weeksafteroperation,therewassignificantlystatisticaldifference(P<0.05)inCAhetweenthetwogroupswithimageanalysissystem.Butat6weeksafteroperation,thebonedensitydiffere

7、ncewasnotstatisticallysignificant(P>0.05).Histologicalslicesintheexperimentalgroup13showedthattheabsorption,organizationofhematoma,theformationoftrabeculaeandmatrixprecededthoseinthecontrolgroup.Thereweremorefibroblast,osteoblastsandcallusintheexperimen

8、talgroup.［Conclusion］Cell-scaffoldcomplexcomposedwithBMCsandβ-TCPstimulatedbyLIPUScanrepairbonedefectmoreeffectivelythanthatwithoutLIPUSstimulationattheearlystageofhealing,buttheeffecttendtodecreaseatthelates