中性粒细胞趋化因子在轻、重症急性胰腺炎大鼠胰腺组织中的表达变化

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1、中性粒细胞趋化因子在轻、重症急性胰腺炎大鼠胰腺组织中的表达变化作者：徐萍徐岷，周国雄，黄介飞，张弘，魏群，江枫，肖明兵【摘要】目的：探讨中性粒细胞趋化因子(cytokineinducedneutrophilchemoattractant,CINC)在轻症急性胰腺炎(mildacutepancreatitis,MAP)向重症急性胰腺炎(severeacutepancreatitis,SAP)演变过程中的作用。方法：分别采用腹腔注射雨蛙肽和胆胰管逆行注射5%牛磺胆酸钠建立大鼠轻症、重症急性胰腺炎模型。雄性SD大鼠随机分为雨蛙肽组(18只)、生理盐水组(18只)、牛磺胆酸钠组(24只)和手术对

2、照组(24只)，检测各组不同时间点血清淀粉酶并观察胰腺组织病理学改变，用免疫组织化学法和半定量逆转录聚合酶链反应(RTPCR)法检测胰腺组织中CINC蛋白和CINCmRNA表达的变化情况。结果：雨蛙肽组6，12h胰腺组织中CINC蛋白和CINCmRNA表达明显高于生理盐水组(P<0.05)，且表达与胰腺组织的病理积分呈正相关;24h与生理盐水组比较无明显差异。牛磺胆酸钠组与手术对照组相比，大鼠的血清淀粉酶明显增高、胰腺组织学改变明显;术后1h胰腺腺泡细胞CINC蛋白和CINCmRNA的表达高于生理盐水组，随着时间的延长表达逐渐增强(P<0.05，P<0.01)。结论：C

3、INC可能在MAP向SAP演变过程中起到重要作用。13【关键词】急性胰腺炎;中性粒细胞趋化因子;大鼠　[Abstract]Objective:Toinvestigatetheroleofcytokineinducedneutrophilchemoattractant(CINC)inthecourseofexchangefrommildacutepancreatitis(MAP)tosevereacutepancreatitis(SAP).Methods:Acutepancreatitismodelwasinducedbyintraperitonealinjectionofcerulein

4、orretrogradeinfusionof5%sodiumtaurocholateintothebilipancreaticductinSDrats.SDratswererandomlydividedintofourgroups：ceruleingroup(eighteen),salinecontrolgroup(eighteen),sodiumtaurocholategroup(twentyfour)andoperationalcontrolgroup(twentyfour).Serumamylaselevelandthehistologicalscoreofpancreaswere

5、measuredatdifferenttimepointineverygroup.ExpressionofpancreaticCINCproteinsandCINCmRNAweredeterminedbyimmunohistochemistryandsemiquantitativereversetranscriptasepolymerasechainreaction(RTPCR),respectively.Results:PancreaticCINCproteinsandCINCmRNAwereincreasedinceruleingroupincomparisonwithsaline

6、controlgroup6,12hoursfollowingthefirstinjection(P<0.05).ExpressionofCINCwascorrelatedwiththehistologicalscoreofpancreas.Nosignificantdifferencewasfoundbetweenceruleingroupandsalinecontrol13groupafter24hours.Serumamylaseandthehistologicalscoreofsodiumtaurocholategroupweresignificantlyhigherthant

7、hoseofoperationalcontrolgroup.ExpressionofCINCproteinsandCINCmRNAinpancreaticacinarcellsinsodiumtaurocholategroup1hourafteroperationweresignificantlyhigherthanthoseofoperationalcontrolgroup.ExpressionofCINCweregr