不同品系来源的骨髓间充质干细胞对小鼠肾缺血再灌注损伤作用的对比性研究

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1、不同品系来源的骨髓间充质干细胞对小鼠肾缺血再灌注损伤作用的对比性研究作者：丁国永郭丽陈强卢日峰孟繁凯凌翎李鹏范洪学【摘要】目的探讨不同品系的骨髓间充质干细胞（BMSCs）对各自小鼠肾缺血再灌注损伤是否有促修复作用，为临床治疗肾脏疾病提供思路。方法取健康3w龄C57BL/6J小鼠和ICR小鼠的骨髓细胞悬液，进行BMSCs体外扩增培养。将8w龄C57BL/6J小鼠和ICR小鼠各随机分为假手术组、平行对照组和细胞移植组，每组12只小鼠。建立肾缺血再灌注损伤模型，将BMSCs移植到细胞移植组小鼠中，平行对照组注射生理盐水。2w后

2、，检测肾功能和肾形态学变化，观察不同品系的BMSCs对各自小鼠肾缺血再灌注损伤是否有促修复作用。结果分离培养的BMSCs增殖旺盛，纯度较高，且均质性和稳定性好。BMSCs移植C57BL/6J小鼠后，细胞移植组小鼠整体状态良好，与平行对照组相比肾功能指标明显改善（P＜0.05），组织形态学好转。而BMSCs移植ICR小鼠后，细胞移植组小鼠整体状态差，肾功能指标与平行对照组相比没有差异或者明显高于平行对照组（P＞0.05），肾病理性改变没有得到改观。结论经尾静脉移植BMSCs后，可提高肾缺血再灌注损伤后宿主C57BL/6J小

3、鼠的恢复；但不能改善ICR小鼠肾缺血再灌注造成的损伤，甚至加剧了ICR小鼠肾损伤的程度。【关键词】骨髓间充质干细胞；肾缺血再灌注；修复11【Abstract】ObjectiveToexplorewhetherbonemarrowmesenchymalstemcells(BMSCs)fromdifferentstrainmicecanpromoterepairingrenalischemiareperfusion(I/R)injuryandprovideamethodforthetreatmentofrenaldisea

4、ses.MethodsBMSCswereculturedandexpandedfromhealthy3weekoldC57BL/6JmiceandICRmiceinvitro,respectively.8weekoldC57BL/6JmiceandICRmicewererandomlydividedintosham,parallelcontrolandcelltransplantationgroups,and12miceineachgroup.TherenalI/Rinjurymodelwasestablished.

5、BMSCsweretransplantedintocelltransplantationgroupmiceviathetailveinandsalinewasinjectedintoparallelcontrolgroup.Then,renalfunction,morphologicchangesandrenalpathologychangesweredetectedafter2weeks.Therecoveryofkidneyofdifferentstrainsmicewereobserved.ResultsBMSCsno

6、tonlyhadahighpurification,homogeneityandstability,butalsohadunlimitedproliferation.The57BL/6Jmiceintransplantationgroupappearedthegoodstate,andtheindexesofrenalfunctionweresignificantlyimproved(P＜0.05),therenalhistomorphologybecamebetter.TheICRmiceintransplantation

7、groupappearedthepoorstate,theindexesofrenalfunctionwerenotdifferentfromthoseincontrolgroup,andsomeindexeswere11significantlyhigherthanthoseofcontrolgroup(P＞0.05),therenalpathologicalchangeswerenotimproved.ConclusionsTherecoveryofrenalI/Rinjuryinthehostisimprovedaft

8、ertransplantationofBMSCsintoC57BL/6Jmicebytailvainandrenalinjuryisimproved.ButBMSCscannotimproveICRmicerenalI/Rinjury,orevenexacerbatetheextentof