不同来源间充质干细胞治疗小鼠放射性肺损伤的研究

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1、分类号密级UDC保密期限博士学位论文题目不同来源间充质干细胞治疗小鼠放射性肺损伤的研究作者姓名申戈指导教师张伟京主任医师培养单位军事医学科学院附属医院专业名称免疫学论文提交日期2012年06月15日学位授予单位中国人民解放军军事医学科学院答辩委员会主席郭宁中国人民解放军军事医学科学院制目录目录1缩略语2中文摘要3英文摘要

2、6前言10第一部分小鼠不同来源间充质干细胞治疗小鼠放射性肺损伤的研究14材料与方法14实验结果16讨论19小结一22第二部分小鼠胚胎来源间充质干细胞治疗小鼠放射性肺损伤的研究

3、23材料与方法23实验结果26讨论33小结二35第三部分人源性不同来源间充质干细胞治疗小鼠放射性肺损伤的研究36材料与方法36实验结果38讨论

4、46小结三48讨论49结论51参考文献52综述一59综述二71博士期间发表的相关论文81个

5、人简历91致谢921英文缩略词表英文缩写英文全名中文译名ACEAngiotensinconveningenzyme血管紧张素转换酶AGMaorta-gonad-mesonephros主动脉-性腺-中肾AMSCAmnioticmesenchymalstemcells羊膜间充质干细胞ATMataxiatelangiectasiamutated共济失调毛细血管扩张突变CBACytometricBeadArray流式

6、细胞术微球阵列法CMConditionedmedium条件培养液DAdorsalaorta胚胎背主动脉DMEMDulbecco’smodifiedEagle’smediumDulbecco改进的Eagle培养基DMSOdimethylsulfoxide二甲基亚砜E0.5embryoday0.5孕0.5天ELISAenzyme-linkedimmunosorbentassay酶联免疫法FACSfluorescence-activatedcellsorter流式细胞仪FBSfetalbovineserum胎牛血清ICAMInt

7、er-CellularAdhersionMolecule细胞间粘附分子IL-6Interleukin-6白细胞介素-6KL-6KrebsvondenLungen-6肺上皮特异蛋白MCP-1Monocytechemotacticprotein-1单核细胞趋化蛋白-1MEMMinimumessentialmedium极限必需培养基mRNAmessengerribonucleicacid信使核糖核酸MSCsMesenchymalstemcells间充质干细胞RILIRadiationinducedlunginjury照射所致肺损

8、伤SPSurfactantProteins肺表面活性蛋白TGF-βtransforminggrowthfactor-β转化生长因子βTNF-αtumornecrosisfactoralpha肿瘤坏死因子αUMSCumbilicalcordmesenchymalstem脐带间充质干细胞cells2中文摘要