与氨基反应的荧光探针

《与氨基反应的荧光探针》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。

1、与氨基反应的荧光探针HighlightsofRhodamineandTRITCLabelingKits:·Fast–labelandpurifyantibodyinaboutonehour·Amine-specificlabeling–theNHS-esterorisothiocyanateefficientlylabelsantibodiesandotherpurifiedproteinsatprimaryamines(lysinesidechains)·Optimizedprocedure–followingthestandardprotoco

2、lresultsinantibodieswithexcellentdye:proteinratiosforoptimumactivityandfluorescence·Single-usefluors–noneedtoweightinyamountspowder;kitscontainsingle-usevialsofreagent·Efficientpurification–kitsincludepurificationresinandeasy-to-usespincolumns,ensuringrapidandefficientremovalo

3、fnon-reacteddyeandexcellentproteinrecoveryApplicationsforRhodamineLabelingReagents:·Labelantibodiesforuseasimmunofluorescentprobes·Labeloligonucleotidesforhybridizationprobes·DetectproteinsingelsandonWesternblotsPropertiesofRhodamineDyes:ThermoScientificPierceRhodamineDyesarem

4、ixturesofisomerswithreactivegroupsattachedatthe5-and6-positionsofthebottomring.Thepropertiesoftheseisomersareindistinguishableintermsofexcitationandemissionspectra,andforproteinapplicationsthereisnoneedtoisolateaspecificisomer.TRITCisthebasetetramethylrhodaminemoleculefunction

5、alizedwithanisothiocyanatereactivegroup(–N=C=S)atoneoftwohydrogenatomsonthebottomringofthestructure.Thisderivativeisreactivetowardsprimaryaminegroupsonproteins,peptidesandotherbiomolecules.NHS-RhodamineisactivatedwiththeN-hydroxy-succinimidyl-ester(NHSester)functionalgroup.Com

6、paredtoTRITC,theNHS-esterderiviativehasgreaterspecificitytowardprimaryaminesinthepresenceofothernucleophilesandresultsinamorestablelinkagefollowinglabeling.TexasRed*SulfonylChlorideisalong-wavelengthderivativeofrhodaminethatismodifiedwithsulfonylchlorideforreactiontoprimaryami

7、nes.WerecommendDyLight594NHSEsterasanalternativetoTexasRedSulfonylChloridebecauseitisbrighter,morephotostableandhasamorespecificlabelingchemistrythatcanbeusedatphysiologicpH.AMCA(aminomethylcoumarinacetate)isabluefluorescentdyewhosereactivederivativesareusedascontrastingprobes

8、fordoubleandtriplelabelinginimmunofluorescencemicroscopy,arra