与氨基反应的荧光探针

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1、与氨基反应的荧光探针HighlightsofRhodamineandTRITCLabelingKits:·Fast–labelandpurifyantibodyinaboutonehour·Amine-specificlabeling–theNHS-esterorisothiocyanateefficientlylabelsantibodiesandotherpurifiedproteinsatprimaryamines(lysinesidechains)·Optimizedprocedure–followingthestandardprotoc

2、olresultsinantibodieswithexcellentdye:proteinratiosforoptimumactivityandfluorescence·Single-usefluors–noneedtoweightinyamountspowder;kitscontainsingle-usevialsofreagent·Efficientpurification–kitsincludepurificationresinandeasy-to-usespincolumns,ensuringrapidandefficientremova

3、lofnon-reacteddyeandexcellentproteinrecoveryApplicationsforRhodamineLabelingReagents:·Labelantibodiesforuseasimmunofluorescentprobes·Labeloligonucleotidesforhybridizationprobes·DetectproteinsingelsandonWesternblotsPropertiesofRhodamineDyes:ThermoScientificPierceRhodamineDyesa

4、remixturesofisomerswithreactivegroupsattachedatthe5-and6-positionsofthebottomring.Thepropertiesoftheseisomersareindistinguishableintermsofexcitationandemissionspectra,andforproteinapplicationsthereisnoneedtoisolateaspecificisomer.TRITCisthebasetetramethylrhodaminemoleculefunc

5、tionalizedwithanisothiocyanatereactivegroup(–N=C=S)atoneoftwohydrogenatomsonthebottomringofthestructure.Thisderivativeisreactivetowardsprimaryaminegroupsonproteins,peptidesandotherbiomolecules.NHS-RhodamineisactivatedwiththeN-hydroxy-succinimidyl-ester(NHSester)functionalgrou

6、p.ComparedtoTRITC,theNHS-esterderiviativehasgreaterspecificitytowardprimaryaminesinthepresenceofothernucleophilesandresultsinamorestablelinkagefollowinglabeling.TexasRed*SulfonylChlorideisalong-wavelengthderivativeofrhodaminethatismodifiedwithsulfonylchlorideforreactiontoprim

7、aryamines.WerecommendDyLight594NHSEsterasanalternativetoTexasRedSulfonylChloridebecauseitisbrighter,morephotostableandhasamorespecificlabelingchemistrythatcanbeusedatphysiologicpH.AMCA(aminomethylcoumarinacetate)isabluefluorescentdyewhosereactivederivativesareusedascontrastin

8、gprobesfordoubleandtriplelabelinginimmunofluorescencemicroscopy,arra