与氨基反应的荧光探针

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1、与氨基反应的荧光探针HighlightsofRhodamineandTRITCLabelingKits:·Fast–labelandpurifyantibodyinaboutonehour·Amine-specificlabeling–theNHS-esterorisothiocyanateefficientlylabelsantibodiesandotherpurifiedproteinsatprimaryamines(lysinesidechains)·Optimizedprocedure–followingthestandardprotocolresultsinantibod

2、ieswithexcellentdye:proteinratiosforoptimumactivityandfluorescence·Single-usefluors–noneedtoweightinyamountspowder;kitscontainsingle-usevialsofreagent·Efficientpurification–kitsincludepurificationresinandeasy-to-usespincolumns,ensuringrapidandefficientremovalofnon-reacteddyeandexcellentprotein

3、recoveryApplicationsforRhodamineLabelingReagents:·Labelantibodiesforuseasimmunofluorescentprobes·Labeloligonucleotidesforhybridizationprobes·DetectproteinsingelsandonWesternblotsPropertiesofRhodamineDyes:ThermoScientificPierceRhodamineDyesaremixturesofisomerswithreactivegroupsattachedatthe5-an

4、d6-positionsofthebottomring.Thepropertiesoftheseisomersareindistinguishableintermsofexcitationandemissionspectra,andforproteinapplicationsthereisnoneedtoisolateaspecificisomer.TRITCisthebasetetramethylrhodaminemoleculefunctionalizedwithanisothiocyanatereactivegroup(–N=C=S)atoneoftwohydrogenato

5、msonthebottomringofthestructure.Thisderivativeisreactivetowardsprimaryaminegroupsonproteins,peptidesandotherbiomolecules.NHS-RhodamineisactivatedwiththeN-hydroxy-succinimidyl-ester(NHSester)functionalgroup.ComparedtoTRITC,theNHS-esterderiviativehasgreaterspecificitytowardprimaryaminesinthepres

6、enceofothernucleophilesandresultsinamorestablelinkagefollowinglabeling.TexasRed*SulfonylChlorideisalong-wavelengthderivativeofrhodaminethatismodifiedwithsulfonylchlorideforreactiontoprimaryamines.WerecommendDyLight594NHSEsterasanalternativetoTexasRedSulfonylChloridebecauseitisbrighter,morephot

7、ostableandhasamorespecificlabelingchemistrythatcanbeusedatphysiologicpH.AMCA(aminomethylcoumarinacetate)isabluefluorescentdyewhosereactivederivativesareusedascontrastingprobesfordoubleandtriplelabelinginimmunofluorescencemicroscopy,arra