发育期大鼠反复痫性发作后远期学习记忆障碍及海马神经元可塑性的研究

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1、发育期大鼠反复痫性发作后远期学习记忆障碍及海马神经元可塑性的研究作者：罗平香,唐洪丽单位：东南大学临床医学院儿科学教研室，江苏南京210009【摘要】目的探讨发育期大鼠反复痫性发作对大鼠成年后学习记忆及海马神经元可塑性的影响。方法生后10dSD大鼠32只，随机分为实验组16只和对照组16只。实验组用戊四氮(PTZ)反复腹腔注射5d，制成反复痫性发作模型，对照组腹腔注射等量生理盐水。在生后第60天用Morris水迷宫评价大鼠的学习记忆功能，用RTPCR方法检测大鼠海马神经元可塑性标志物神经生长相关蛋白43(GAP43)mRNA的表达

2、变化。结果与对照组比较,实验组在Morris水迷宫测试中平均逃避潜伏期明显延长(P<0.05),原平台所在象限的游泳时间明显缩短(P<0.05);海马组织GAP43mRNA的表达显著增多(P<0.05)。结论发育期大鼠反复痫性发作可引起远期学习记忆损害及海马神经元激活和突触重建等神经元可塑性改变，而GAP43表达增多可能是学习记忆功能和可塑性变化的分子机制。【关键词】癫痫;发育;学习记忆;神经生长相关蛋白43;可塑性Longtermeffectsonlearningandmemoryandhippocamusn

3、europlasticityinimmatureratsfollowingrecurrentseizuresLUOPingxiang,TANGHongli(DepartmentofPediatrics,SchoolofClinicaldicine,SoutheastUniversity,Nanjing210009,China)bstract:ObjectiveToexploretheeffectsofrecurrentseizuresinimmatureratsonlearningandmemoryandhippocamusneur

4、oplasticitywhentheanimalswereinadulthood.MethodsRatsatpostnatalday10(n=32)weredividedintoexperimentalgroup(n=16)andcontrolgroup(n=16).Ratsintheexperimentalgroupweretreatedwithpentylenetetrazol(PTZ)dailybyintraperitonealinjectionfor5consecutivedaystoinducerecurrentseizure

5、andratsinthecontrolgroupwereinjectedwithnormalsalinefor5consecutivedays.Allratsatpostnatalday60weretestedforspatiallearningandmemorybyMorriswatermazetask.TheexpressionsofGAP43mRNA,themarkerforneuroplasticityinhippocampusweredetectedbyreversetranscriptionpolymerasechainr

6、eaction.ResultsIntheexperimentalgroup,themeanescapelatencyofsearchingtheplatformsignificantlyprolonged(P<0.05)andswimmingtimeintheoriginalplatformregionsignificantlyshortened(P<0.05)inMorriswatermaze.TheexpressionofGAP43mRNAinhippocampusintheexperimentalgroupwassi

7、gnificantlyincreasedthanthatinthecontrolgroup(P<0.05).ConclusionThesefindingsindicatethatrecurrentseizureinimmatureratshavelongtermdetrimentaleffectsonlearningandmemoryandchangesofhippocamusplasticity,suchassynapticreoganizationandtheactionofneuron.GAP43increasingma

8、ybethemolecularmechanismsforlearningandmemoryandhippocamusneuroplasticity.　　Keywords:epilepsy;developme