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ID:33131782
大小:3.56 MB
页数:37页
时间:2019-02-21
《卒中后抑郁大鼠学习记忆障碍研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、浙江大学硕士学位论文卒中后抑郁大鼠学习记忆障碍研究姓名:毛琳申请学位级别:硕士专业:神经病学指导教师:罗本燕20120301浙江大学硕士学位论文中文摘要卒中后抑郁大鼠学习记忆障碍研究浙江大学医学院神经病学2007级同等学历硕士研究生毛琳导师罗本燕教授中文摘要目的对比脑缺血与慢性应激所致学习记忆障碍及海马病变的强弱,为临床改善脑卒中后抑郁(Post.s仃0kedep他ssionPSD)提供参考.实验方法40只成年雄性SD大鼠平均分为4组:对照组.抑郁(应激)组,卒中(缺血)组与PSD(缺血加应激)组,卒中处
2、理采用改良的选择性大脑中动脉栓塞术;抑郁处理采用连续3周的慢性不可预见性温和应激;Morris水迷宫实验评价依赖海马的学习记忆功能;免疫组织化学染色及半定量RT-PCR观察海马CA3区脑源性神经营养因子(B豫in.deriveneuron℃phicflactor,BDNF)的表达变化.结果缺血或应激均可使大鼠学习功能明显下降,表现为与同时点对照组比较,逃避潜伏期显著延长,二者的综合作用更明显.慢性应激对学习功能的影响强于脑缺血损伤.缺血或抑郁减弱记忆功能,但二者的作用差异无统计学意义.与对照相比,缺血显著
3、增强海马CA3区BDNF的表达(27.0士2.5与20.1士2.1),应激降低BDNF的表达(15.2士1.8与20.1士2.1),二者综合作用仍显著降低BDNF的表达(8.2士1.5),差异均具有统计学意义(F=52.87.P4、经营养因子;学习记忆UIResearchonthecognitiVeimpairmentoflearningandobjectiVememoryinratswithpost-strokedepressionMedicalCo¨ege,ZheJiangUniVeI.si锣PostgraduateSuperVisorLinMaOProf.YranBenLuoAbstractTbcomparet量leintensi哆ofcognitiVeimpaimlentofle锄ingandmemoq弛dtheleVelo5、fpathologicaIlesioninhippocampusinducedbyiSchemiaorch∞nics仃essforamo他ValuabIeguidanceinthe臼℃atnlentofpoSt—Strokedeprcssion(PSD).MethOdSFortymaIeadultSD豫tS、代咒diVidedmediallyinto4groups:con仃l{0l,dep他ssion,Stroke锄dPSD.Animalsin3仃eatmentgmupsweresubjectedresp6、ectivelyt0觚operationofmodi6edselectiVemiddIecercb随lanc呵0cclusionoraprocedureofcontinuous3。w∞kchronicunp捌ictablemildsn℃ssoracombinedprog跚nofthet、Ⅳo讹atments.Mo盯iswaterm北ew懿锄p10yedt0嬲∞sshippc.campus·dependentle栅ing锄dmemo哕mnction锄dthebmin.derivedneumtmphicfac7、tor(BDNF)exp他ssi彻w嬲detectedbyimm岫ohistochemicals协ininginCA3a嗽鲫dthemRNAampIi行cationthrou曲∞mi.quantitatiVereVe赋心柚ScriptionpoIymemsechainr%ction(RT.PCR).ResuItsBothchronics仃℃ssors锄dischemiacouldsign讯c卸tlydec他a辩thele姗ing柚dmemo哆向nctionin眦sliketheescapelatencyi8、nthepe—b肌蛳ceoftIIeMorrisw乏她rma职tcstcomp躺dwiththecon臼01.The蚰孵ssgmupw觞rel觚cdprcfcrcntialIyt0amo他s“e陀detcriomtionintheIe拥ing向nctionbutnotStatisticallyinthememo叫IV浙江大学硕J:学位论文英文摘要loss鹊comparcdt0ischemiagroup.T.hecognitiV
4、经营养因子;学习记忆UIResearchonthecognitiVeimpairmentoflearningandobjectiVememoryinratswithpost-strokedepressionMedicalCo¨ege,ZheJiangUniVeI.si锣PostgraduateSuperVisorLinMaOProf.YranBenLuoAbstractTbcomparet量leintensi哆ofcognitiVeimpaimlentofle锄ingandmemoq弛dtheleVelo
5、fpathologicaIlesioninhippocampusinducedbyiSchemiaorch∞nics仃essforamo他ValuabIeguidanceinthe臼℃atnlentofpoSt—Strokedeprcssion(PSD).MethOdSFortymaIeadultSD豫tS、代咒diVidedmediallyinto4groups:con仃l{0l,dep他ssion,Stroke锄dPSD.Animalsin3仃eatmentgmupsweresubjectedresp
6、ectivelyt0觚operationofmodi6edselectiVemiddIecercb随lanc呵0cclusionoraprocedureofcontinuous3。w∞kchronicunp捌ictablemildsn℃ssoracombinedprog跚nofthet、Ⅳo讹atments.Mo盯iswaterm北ew懿锄p10yedt0嬲∞sshippc.campus·dependentle栅ing锄dmemo哕mnction锄dthebmin.derivedneumtmphicfac
7、tor(BDNF)exp他ssi彻w嬲detectedbyimm岫ohistochemicals协ininginCA3a嗽鲫dthemRNAampIi行cationthrou曲∞mi.quantitatiVereVe赋心柚ScriptionpoIymemsechainr%ction(RT.PCR).ResuItsBothchronics仃℃ssors锄dischemiacouldsign讯c卸tlydec他a辩thele姗ing柚dmemo哆向nctionin眦sliketheescapelatencyi
8、nthepe—b肌蛳ceoftIIeMorrisw乏她rma职tcstcomp躺dwiththecon臼01.The蚰孵ssgmupw觞rel觚cdprcfcrcntialIyt0amo他s“e陀detcriomtionintheIe拥ing向nctionbutnotStatisticallyinthememo叫IV浙江大学硕J:学位论文英文摘要loss鹊comparcdt0ischemiagroup.T.hecognitiV
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