pten和p53蛋白异常表达与子宫内膜异位症恶变的关系

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1、PTEN和p53蛋白异常表达与子宫内膜异位症恶变的关系【摘要】目的探讨PTEN基因和p53基因蛋白表达异常与子宫内膜异位症（简称内异症）恶变的关系以及两者在其发生发展过程中的相互作用。方法应用免疫组化PV9000二步法检测23例内异症相关卵巢癌(EAOC)、10例不典型内异症(aEMs)和20例典型卵巢内异症(EMT)组织中PTEN和p53基因蛋白表达情况。结果EAOC中PTEN、p53基因蛋白表达缺失率分别为78.26%和17.39%，前者高于EMT组(5%)和aEMs组(20%)，后者低于EMT组(85%)和aEMs组(70%)。EAOC中PTEN、p53基因蛋白表达缺失率与EMT

2、组相比差异有高度统计学意义(P＜0.01)，与aEMs组相比差异有统计学意义(P＜0.05)。EAOC中PTEN、p53基因蛋白表达缺失率均与临床分期、病理分级、是否伴有转移密切相关(P＜0.05)，与年龄、组织学类型无关(P＞0.05)，不同的是，PTEN在临床分期早、组织分化好、无转移的EAOC中的蛋白表达缺失率明显低于临床分期晚、组织分化差和(或)伴转移者，而p53蛋白表达相反。PTEN和p53基因的蛋白表达呈负相关。结论PTEN蛋白表达缺失和p53蛋白过表达与内异症相关卵巢癌及内异症恶变的发生发展密切相关。【关键词】子宫内膜异位症；卵巢肿瘤；不典型增生；PTEN基因；p53基因；

3、免疫组化CorrelationoftheproteinexpressionofPTENandp53withmalignanttransformationofendometriosisMAXiaoping,ZHENZonghui,XUBing,DAIShuzhen,ZHAOPeng,WEIZhimin(Dept.ofGynecology,AffiliatedHospitalofQingdaoUniversity)Abstract:Objective:ToinvestigatetherelationshipbetweenproteinexpressionsofPTENandp53ge

4、nesandmalignanttransformationofendometriosis.Methods:Twentythreeendometriosisassociatedovariancancers(EAOC),10atypicalhyperplasiaendometriosisofovaryand20typicalendometriosistissueswereusedtodetecttheproteinexpressionsofPTENandp53genesusingimmunohistochemistryPV9000method.AmongthetwentythreeEA

5、OCsamples,ninewereendometrioidcarcinomas,fourteenwereclearcellcarcinomas,andfourofthemdemonstratedcontinuitybetweencancerandendometriosistissue.Results:EighteenofthetwentythreeEAOCcases(78.26%)andfour(17.39%)hadPTENandp53proteinexpressiondeletion,withthedeletionratesofPTENhigherandp53lowerthanth

6、oseofEMTandaEMsrespectively.TheproteinexpressiondeletionratesofPTENinEMTandaEMswere5%and20%,butthoseofp53were85%and70%respectively.TherewassignificantlydifferencewhenEAOCwascomparedwithEMToraEMs(P＜0.05).TheratesofproteinexpressionofPTEN/p53ofEAOCwerenotrelativetoagedifferenceandhistologicaltype(P

7、＞0.05),andbuthadsignificantrelationshipwithclinicalstage,pathologicalgradeoraccompaniedmetastasis(P＜0.05).TherateofproteinexpressiondeletionofPTENofEAOCwhichwasinearlyclinicalstage,welldifferentiatedandnotaccompaniedb