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1、SR—AⅡ和CD36的表达水平与AGEs及糖尿病并发症的相关性研究?168?[文章一号】1000-4718(2007)01-0168—05ChineseJournalofPathophysiology2唧堑();=!SR—AⅡ和CD36的表达水平与AGEs及糖尿病并发症的相关性研究陈万群,骆婕,彭锐,吴颜晖,莫宏波,冯烈,李菊香,刘誉(暨南大学医学院生化教研室,临床医学系,'附属华侨医院,广东广州510632)[摘要】目的:分析糖尿病患者白细胞中清道夫受体sR—AⅡ和cD36的表达水平与血浆晚期糖基化终产物(AGE~)浓度以及
2、糖尿病并发症之间的相关性.方法:收集具有合并症的糖尿病患者抗凝血78例,用荧光分光光度法测定血浆AGEs浓度.用Trizol法提取白细胞RNA,再以RT—PCR法测定SR—AⅡ和CD36的相对表达水平.结果:在测定的糖尿病各合并症中,SR—AII在糖尿病性肾病中表达水平最高,在脂肪肝患者中最低;CD36在脂肪肝患者中最高,在冠心病患者中最低.在高血压患者中这2种受体的表达水平均比较高,但在白内障患者中2种受体的表达相对较低.血浆AGEs浓度与白细胞中CD36表达水平呈负相关(r=一0.89,P<0.01),但与SR—AI
3、I的表达为正相关(r=0.82,P<0.05).结论:血浆高浓度AGEs刺激白细胞中SR—AⅡ表达增加,糖尿病性肾病和脂肪肝分别与SR—AⅡ,CD36的表达增加有密切关系,部分糖尿病患者CD36的表达较低可能是导致他们血浆AGEs水平过高的原因之一.【关t词】CD36;糖尿病;糖基化终产物,高级;受体,清道夫【中圈分类号】R363【文献标识码】AExpressionofSR.--AHandCD36inwhitebloodcellscorrelatewithplas.msAGEslevelsanddiabeticcompl
4、ications—CHENWan—qun,LUOJie,PENGR_ui—rui,WUYan—hui,MOHong—bo,FENGLie'.LIJu—xiang3.LIUYu(ofBiochemistry";DepartmentofClinicalMedicine,MedicalCollege,AffiliatedOversea—ChineseHos-p/m/,J/nan￡,Gl删lg:dl'610623,Ch/na.E—mad:uZ'u05@126.∞,,1)[ABSTRACT]AIM..Toinvestigatetheco
5、rrelationoftheexpressionofscavengerreceptorsSR—AIIandCD36inwhitebloodcells(WBCs)withtheplasmalevelsofadvancedglyeosylationendproducts(AGF~)anddifferentdiabeticcomplications.METHODS:Bloodsampleswel'~collectedfrom78patientswithdiabeticcomplications.Thelevelsofplas-眦AG
6、Esweredeterminedbyusingspectrofluorimetry.RNAinWBCsW88extractedwithTrimlreagentandthemRNAlev-elsofSR—AⅡandCD36we豫determinedbyRT—PCR.RESIⅡ:Inthepatientstested,themRNAlevelofSR—AI1w船foundtobethehighestinthosewithdiabeticnephropathy.and.1owestinthosewithfattyliver.Thee
7、xpressionofCD36W88foundtobethehighestindiabeticpatientswithfattyliverandlowestinthosewithcoronaryheartdisease.Theexpression缸bethreceptorsinWBCsshowedsignificantlyhigherlevelsindiabeticpatientswithhypertension.andlowerinthoseWitllcataract.TheplasmalevelsofAGEsnegativ
8、elycorrelatedwithmRNAlevelsofCD36(r=一0.89,P<0.01),whilepositivelycorrelatedwithSR—AⅡmRNAlevels(r=一0.82,P<0.05).CONCLUSION:Theincreas
