资源描述：
《14-不同寄主来源的灰葡萄孢对番茄的致病力分化研究》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、14-不同寄主来源的灰葡萄孢对番茄的致病力分化研究不同寄主来源的灰葡萄孢对番茄的致病力分化研究李喜玲高智谋*李艳梅曹中革陈伟汪世军?安徽农业大学植物保护学院合肥230036摘要：从安徽合肥、蚌埠、长丰、和县等市、县的番茄、辣椒、草莓、葡萄等发病寄主上分离鉴定获得18个灰葡萄孢Botrytiscinerea菌株，采用菌丝块创伤接种法，分别测定了上述不同寄主来源的灰葡萄孢菌对番茄果实和叶片的致病力。结果表明，所有供试菌株接种番茄果实后均引起发病，但不同菌株所致病斑的平均直径有显著差异，显示灰葡萄孢菌株间
2、对番茄果实的致病力存在明显分化。按照在番茄果实上所致病斑的平均直径大小可将供试菌株致病力划分为较强、中等和较弱3种类型。总体来说，来自番茄的菌株对番茄果实的致病力较强，来自草莓、葡萄和辣椒的菌株对番茄果实的致病力较弱，但来自相同寄主的菌株间致病力也存在差异，菌株致病力差异与菌株地域来源无明显相关。供试灰葡萄孢菌株接种番茄叶片后，除CF1外，均可引起番茄叶片发病，但不同菌株所致番茄叶片病斑的平均直径也有显著差异；供试菌株对番茄叶片的致病力差异与菌株的寄主和地域来源无显著相关。关键词：番茄灰霉病，病斑平
3、均直径，致病类型，地域来源DifferentiationofpathogenicityofBotrytiscinereastrainsfromdifferenthoststotomatoLIXi-LingGAOZhi-Mou?LIYan-MeiCAOZhong-GeCHENWeiWANGShi-JunCollegeofPlantProtection,AnhuiAgriculturalUniversity,Hefei230036,ChinaAbstract:EighteenisolatesofBotr
4、ytiscinereawereobtainedfromthediseasedplanttissuecollectedinHefei,Bengbu,ChangfengandHexianinAnhuiprovince,bymeansoftissueisolatingmethod.ThepathogenicityoftheisolatesofB.cinereafromdifferenthoststothefruitsandleavesoftomatowereinvestigatedbyapplyingwo
5、undinoculationwithmycelialblocks.Theresultsshowedthatallofthetestedisolatescausedgreymouldontomatofruits,buttherewassignificantdifferenceintheaveragediametersofthelesionscausedbydifferentisolates,suggestingthattherewassignificantdifferentiationinpathog
6、enicityofB.cinereastrainstotomatofruitsamongisolates.Accordingtotheaveragediametersofthelesionsontomatofruits,thepathogenicityoftheallisolateswasclassifiedintothreecategories:strong,intermediateandweak.Ingeneral,theisolatesfromtomatoweremorestronglypat
7、hogenictotomatofruitsthantheisolatesfromstrawberry,grapeandcapsicum.However,therewasdifferenceinpathogenicityamongthedifferentisolatesfromthesamehost,andthepathogenicitydifferencewasnotobviouslyrelatedtothelocalitiesofisolates.Afterinoculatingoftomatol
8、eaves,allofthetestedisolatesexceptCF3causedgreymouldontomatoleaves,buttherewassignificantdifferenceintheaveragediametersofthelesionscausedbydifferentisolates;andthedifferenceinpathogenicitytotomatoleaveswasnotobviouslyrelatedtothehostan
