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1、·oProtocolsoStandardProtocoloManual/Datasheet·Cat.#ProductSizeNote3450ProteinMolecularWeightMarker(Low)200lanes3451ProteinMolecularWeightMarker(High)200lanes3452ProteinMolecularWeightMarker(Broad)200lanesDescriptionProteinMolecularWeightMarkersaredesi
2、gnedforuseinSDS-Polyacrylamidegelelectrophoresis.Thereare3kindsofProteinMolecularWeightMarker,Low(Molecularweightrange:14.3-97.2kDa),High(Molecularweightrange:44.3-200kDa)andBroad(Molecularweightrange:6.5-200kDa).Eachproteinisproportionedtoyieldunifor
3、mbandintensitieswhenperformstainingCoomassieBrilliantBlueR-250afterrunonSDSpolyacrylamidegel.5µlof20-folddilutedmarkerisloadedperlaneofSDS-PAGEminigelforstandarduse.Inthiscase,thismarkerissufficientfor200lanes.ElectrophoresisResultLow(Cat.#3450)High(C
4、at.#3451)Broad(Cat.#3452)15%SDS-PAGE7.5%SDS-PAGE5-20%gradientSDS-PAGEStorageProteinMolecularWeightMarker,1MDTT:-20°C5×LoadingBuffer:Storeatroomtemperatureafterused.ComponentProteinMolecularWeightMarker50µl5×LoadingBuffer1ml1MDTT100µlConcentration12µg/
5、µl(Cat.#3450)10µg/µl(Cat.#3451)18µg/µl(Cat.#3452)Form50mMTris-HCl,pH6.81mMEDTA200mMNaCl50%glycerolComponentProteinLow(Cat.#3450)ProteinSourceM.W.(Da)PhosphorylaseBRabbitmuscle97,200SerumAlbuminBovine66,409OvalbuminHeneggwhite44,287CarbonicBovine29,000
6、anhydraseTrypsinInhibitorSoybean20,100LysozymeHeneggwhite14,300High(Cat.#3451)ProteinSourceM.W.(Da)MyosinPig200,000β-galactosidaseE.coli116,000PhosphorylaseBRabbitmuscle97,200SerumAlbuminBovine66,409OvalbuminHeneggwhite44,287Broad(Cat.#3452)Prote
7、inSourceM.W.(Da)MyosinPig200,000β-galactosidaseE.coli116,000PhosphorylaseBRabbitmuscle97,200SerumAlbuminBovine66,409OvalbuminHeneggwhite44,287CarbonicanhydraseBovine29,000TrypsinInhibitorSoybean20,100LysozymeHeneggwhite14,300AprotininBovinepancreas6,5
8、005×LoadingBuffer200mMTris-HCl,pH6.810%SDS0.05%BPB50%GlycerolNote·Allproductsareintendedtobeusedforresearchpurposeonly.Theyarenottobeusedfordrugordiagnosticpurposes,noraretheyintendedforhumanuse.Theyshallnottobeusedproductsasfood,cosmetics,oru
