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1、ClinicalExperience·临床经验胸水脱落细胞DNA含量及VEGF,p53和CEA表达在良恶性胸腔积液鉴别中的意义周 箴1,陈 岗2,宣学金2,张 俭2(1.上海市肺部肿瘤临床医学中心,上海 200030;2.上海交通大学附属胸科医院病理科,上海 200030)[摘要] 目的:探讨检测胸水细胞的DNA倍体以及VEGF、p53、CEA表达鉴别良恶性胸腔积液的价值。方法:73例胸腔积液患者,良性组13例,恶性组60例。图像细胞光度技术(imagecytometry,ICM)进行DNA含量检测,免疫组化Envision法
2、检测VEGF、p53、CEA。结果:良性胸水DNA异倍体率仅占23.1%。恶性胸水的异倍体率占77.8%,两者有统计学差异(P=0.001)。良性胸腔积液患者VEGF、p53、CEA表达率分别为12.5%、0、0。恶性胸腔积液VEGF、p53、CEA表达率分别为17.5%,17.5%和68.4%,与良恶性胸腔积液中CEA的表达相比有统计学差异(P<0.001),VEGF和p53的表达无显著差异(P=0.722,P=0.198)。经DNA倍体联合VEGF、p53、CEA检测,敏感率可达90.4%,特异度87.5%,符合率90%。
3、结论:ICM检测胸水细胞的DNA倍体联合VEGF、p53、CEA表达值得进一步探讨,有可能成为鉴别良恶性胸腔积液的又一方法。[关键词] 肺肿瘤;胸腔积液,恶性;DNA;基因表达;免疫组织化学;诊断,鉴别[中图分类号] R734.2;R730.43 [文献标识码] A [文章编号] 100027431(2007)0220155203ClinicalsignificanceofDNAcontentandVEGF,p53andCEAexpressionsinthedifferentialdiagnosisofmalig2nant
4、andbenignpleuraleffusionsZHOUZhen1,CHENGang2,XUANXue2jin2,ZHANGJian2 (1.ShanghaiLungTumorClinicalMedicalCenter,Shanghai200030,China;2.DepartmentofPathology,ShanghaiChestHospitalofJiaotongUniversity,Shanghai200030,China)[ABSTRACT] Objective:Toinvestigatewhethernuclea
5、rDNAcontentandvascularendothelialgrowthfactor(VEGF),p53andcarcinoembryonicantigen(CEA)expressionshadapotentialvalueindifferentialdiagnosisofmalignantandbenignpleuraleffusions.Methods:Seventy2threepatientswithunilateralpleuraleffusionweredividedinto2groups:benigngrou
6、p(n=13)andmalignantgroup(n=60).TheDNAcontentwasmeasuredbytheimagecytometry(ICM)andthelevelofVEGF,p53andCEAexpressionswerede2tectedbyimmunohistochemicalEnvisionmethod.Results:Theratesofaneuploidinbenignandmalignantpleuralfluidswere23.1%and77.8%,respectively.Thediffer
7、encebetweenthebenignandmalignantpleuraleffusionswassignificant(P<0.001).Immunohis2tochemicalstainingshowedthatthepositiveratesoftheVEGF,p53,andCEAinbenignpleuralfluidwere12.5%,0,and0;inma2lignantpleura1fluidwere17.5%,17.5%,and68.4%,respectively.ThedifferenceinCEAbet
8、weenthebenignandmalignantpleu2raleffusionwassignificant(P<0.001).TheexpressionofVEGFandp53hadnostatisticallydifferencebetweenthebenignandm
