核因子κb活性及环氧化酶2在胃癌及胃炎病变中表达探究

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1、核因子κB活性及环氧化酶2在胃癌及胃炎病变中表达探究【摘要】目的研究胃癌及慢性浅表性胃炎组织中核因子κB（NF-κB）的DNA结合活性，p65及环氧合酶2（COX-2）的表达，通过比较两者表达的差异，从而探讨NF-κB及COX-2在胃癌病程中的意义。方法收集我院65例外科手术胃癌及癌旁组织标本，58例消化内镜慢性浅表性胃炎钳夹组织标本。采用凝胶迁移滞留试验EMSA检测其NF-κBDNA结合活性，免疫组化SABC法测定其NF-κBp65及COX-2蛋白表达水平。结果胃癌标本NF-κBDNA结合活性更高，可见明显核移位现象，与癌旁组织及胃炎组织比较具有统计学差异（P<0．01）

2、,NF-κBp65在胃癌组织，癌旁组织及胃炎中表达阳性率分别为87．7%，10．8%和55．2%，COX-2在胃癌组织，癌旁组织及胃炎中表达阳性率分别为100%，12．3%和53．4%。相关性分析显示胃癌组织中NF-κBp65和COX-2表达呈明显正相关（r＝0．6，P<0．01）。结论NF-κB和COX-2在胃癌组织中阳性表达显著高于癌旁组织和慢性浅表性胃炎组织，且两者存在明显正相关，推测可能通过干预其活性和表达对胃癌的发生发展过程研究有一定帮助。【关键词】核因子κB;环氧化酶2；胃癌；慢性浅表性胃炎12ActivityofNuclearFactor-kappaBandt

3、heExpressionofCyclooxygenase-2inHumanGastricCarcinomaandChronicSuperficialGastritisYANGMing-shi,CHENKen.Departmentofdigestivedisease,theaffiliatedhospitalofGuangdongmedicinecollege,Zhanjiang524001,China【Abstract】ObjectiveStudyontheactivityofNuclearFactor-kappaB(NF-κB）DNA-bindingandtheexpre

4、ssionofp65,Cyclooxygenase-2(COX-2)inhumangastriccarcinomaandChronicSuperficialGastritis.ToexplorethesignificanceofNF-κBandCOX-2inthedevelopmentofgastriccarcinoma.MethodsWecollected65samplesofgastriccarcinomainsurgeryand58samplesofchronicsuperficialgastritiswithgastroscope.TheactivityofNF-κ

5、BDNA-bindingwasdetectedbyelectrophoreticmobilityshiftassay(EMSA).AndtheexpressionofNF-κB-p65andCOX-2weredeterminedbyimmunohistochemicalanalysisofformalin-fixed,paraffin-embeded.ResultsTheactivityofNF-κBDNA-bindingwashigherin12carcinomatissuethanintheothertissuebyEMSA(P<0．05).Thereissignifi

6、cantbetweenthesetwogroups（P<0．05）.ThepositiveexpressionproportionofNF-κB-p65were87．7%incarcinoma,10．8%innormalgastricmucosaand55．2%inchronicsuperficialgastritis.TheexpressionofCOX-2wasdetectedineachgroup.Itwas100%ingastriccarcinoma,wassignificantlyhigherthanthoseinnormalgastricmucosa(12．3%

7、)andchronicsuperficialgastritis(53．4%)（P<0．01）.ConclusionNF-κBandCOX-2wereactivatedingastriccarcinomatissue,anditsexpressionhaspositivecorrelationingastriccarcinomatissue.OurexperimentsuggeststhatCOX-2playsimportantroleinthegastriccarcinomaanddevelopmentofgast