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1、酵母双杂交系统的研究进展（Advancesinyeasttwohybridsystem）*foundationproject:NationalNaturalScienceFoundationofChina(30260029);specialfundofXinjiangConstructionCorps(NKB01SDXN29YX)DepartmentofBiochemistry,Schoolofmedicine,ShiheziUniversity,Shihezi832002,Xinjiang,ChinaAuthor:rahsung(1973-),male,Jia
2、ngsu,lecturer,master,mainlyengagedinGodMolecularbiologystudy.Articlenumber:1001-0580(2005)04-0500-02,CLCnumber:Q78,documentidentifiercode:A[overview]Advancesinyeasttwohybridsystem*Luoxing,HuangJin,ZhangYuhanIn1989,Fields[1]andSongproposedandcreatedadirectyeastAnewgeneticmethodforthed
3、etectionofproteininteractionsincellsyeasttwohybridSystemtwo-hybrid(yeast),alsoknownasinteractiontrap(interac-Tiontrap).Thistechniqueisinvolvedincellcycleanddifferentiation,apoptosis,andsignalingResearchonthefunctionandregulationofgeneandgeneexpressionandthecharacteristicsofproteinsTh
4、edomainisreceivingmoreandmoreattention.Withthedevelopmentandresearchofthistechnology,Yeastsinglehybridization,threehybridandreversedoublehybridizationsystemswerederived.ItsrangeofapplicationExtendedtoproteinsandeggs,endoplasmicreticulum,proteinsandDNA,RNA,andothersmallfractionsThestu
5、dyoftheinteractionofproteinswiththediscoveryofnewproteins,protein-proteininteractionnetworksTheunderstandingofthenetwork,andeventhestudyofthewholeproteome(proteome),hasbeenenormousPromotingrole.1Classicyeasttwohybridsystem1-1principletheestablishmentofyeasttwohybridsystembasedonthere
6、gulationofeukaryotesKnowledgeofthebeginningprocess.ThetranscriptionactivatorsinvolvedinthisgrouparestructurallyIsmodular,whichisoftenrelativelyindependentoftwo(ormorethantwo)Domaincomposition,i.e.,DNAbindingdomain(binding,domain,BD)andtranscriptionActivationdomain(activition,domain,A
7、D).Two,thedomainestablishesspatialrelationsIsthekeytotranscriptionalactivation,butindividualactiondoesnotactivatetranscriptionprocesses.Thegenesofthetargetprotein(prey,ortargetprotein),andtheproteinstobestudied,respectivelySequencesencodingADarecombinedwiththegenesofthedecoy(bait)pro
8、teinandcodin
