hbv血清学定量检测技术及应用

《hbv血清学定量检测技术及应用》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。

1、HBV血清学定量检测技术及应用雅培诊断钱学庆博士HBV血清学定量检测技术及应用1CompanyConfidential©2009AbbottTIMELossofHBeAgLossofHBVDNAAnti-HBe+LossofHBsAgAnti-HBs+ImprovedsurvivalImprovedhistologyTreatmentendpointsdifferforantiviralandimmune-basedtherapies乙型肝炎治疗终点Prof.Marcellin,FranceCommunication/presentatio

2、n“ClinicalstrategiestoCHB–matchingthetreatmenttopatients”PresentationTitleDate2CompanyConfidential©200XAbbott免疫应答引起的抗体Anti-HBc,Anti-HBc-IgMAnti-HBeAnti-HBs病毒标志物HBVDNAHBeAgHBsAgHepatitisB–诊断标志物PresentationTitleDate3CompanyConfidential©200XAbbott两步法检测PresentationTitleDate4Com

3、panyConfidential©200XAbbott两步法可以消除HOOK效应Step1+WASHWASHStep2Sampleantigenfillsallbindingsitesonthesolidphase,butextraantigeniswashedaway.Conjugatelabelbindsnormallytoantigens,samplewillrecordhighvalueondetection,leadingtoflaggingoftheresultneedingdilution.PresentationTitleDat

4、e5CompanyConfidential©200XAbbott一步法和两步法试剂的比较分析1-stepassay2-stepassayStrenghtsEasytoautomatePotentiallybetterprecisionvs2-StepPotentiallyfasterassayInexpensivetodevelop(assay&instrumentoptimization)SuitabletoalmostallassaysExcellentclinicalperformance:NoHigh-Dose-HookeffectNo

5、interferencebyThyroidHormoneAutoantibodiesLessinterferencesbynon-specificbindingWeaknessesMoreriskforclinicallysignificantinterferences,ie.thyroid,microbiology,cancermarkerassays.RiskofHigh-Dose-HookeffectMorecomplextoautomatePotentiallyslowerassaysPotentiallypoorerprecisionM

6、oreexpensivetodevelop(assay&instrumentationoptimization)PresentationTitleDate6CompanyConfidential©200XAbbottARCHITECTHBsAg定量检测PresentationTitleDate7CompanyConfidential©200XAbbottHBsAg的重要临床意义HBsAg是肝细胞受到HBV感染的标志HBsAg清除是肝内HBV感染的免疫控制和抗病毒应答的标志HBsAg清除最接近治愈相比HBVDNA，HBsAg清除能更好地代表疾病

7、的持久缓解HBsAg清除可改善临床预后：降低肝硬化和肝癌的发生率；提高生存率最近的研究报告显示：肝细胞内cccDNA水平（HBV感染细胞的标志）和血清HBsAg水平存在显著相关性目前缺少商业化cccDNA检测试剂；是一种创伤性检测方法，应用受到限制ColombattoP.etal,AntiviralTherapy2006；Werle-Lapostolleetal,Gastroenterology2004；Volzetal,Gastroenterology2007PresentationTitleDate8CompanyConfidential©

8、200XAbbott肝细胞内HBV的生活史AdaptedfromLaiCL,etal.JMedVirol.2000;61:367-373