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1、Graves眼病IgG和细胞因子IFN—γ中国医科大学JChinMedUniv第28卷第1期1999年2月,.Graves眼病IgG和细胞因子IFN一对体外培养人眶后成纤细胞的刺激作用7坠主一圭三.苎滕卫平李静李忠民..(中国医科大学第一临床学院眼科.沈陌ll000I)摘要目的:Graves眼病(GO)的病理组织学特点是球后成纤维细胞产生大量葡萄糖胺聚糖(GAGs)和胶原蛋白沉积,造成眼球竞出.末文从体外实验的角度探讨末病对球后成纤堆细胞的刺激因素.方法:应用10例Graves眼稿,7删Graves病,4俐结带性甲状臃肿伴甲状朦机能亢进症病人和8删正常^IgG和细
2、胞因子IFN—与培养的^日匡后成蚌堆细胞(RF)在体外孵育,观察透明质醢(HA),IV型腔原蛋白产生量和细胞增殖的状态.结果:GO和其他甲状腺疾病病人以及正常凡的IgG都能明显刺激RF台成HA增加,奋蛆之间无明显差异}对Ⅳ型腔原蛋白的台成和RF增殖,各组IgG皆无影响F细胞因子IFN—能够刺斑RF合成HA明显增加,但是却押割IV型腔原蛋白的合成.结论:Graves眼病患者血清中未盅现针对眶后成纤维细胞的特异性自身抗体;IFN一刺激RF产生HA的作用可能与末病病理变化有关工拿竹关键词Grave$艰病F眶后成纤维细胞}免疫球蛋白G{干扰素{遣明质畦,————～————
3、——～StimulatingEffectsofIgGfromthePatientswithGraves'OphthalmopathyandlFN—yon目uma廿RetroorbRalFjbroblastsinVitroChenLei,LiYushu.TengWeiping,etal(Deparm~entofOphthalmology,TheFirstClinicalCollegetChinaMedicalUniversity,Shenyang?110001ABSTRACT0bjecttve:Graves'ophthalmopathy(GO)ischaracter
4、izedhistologicallybyallaccumulationofglycosaminoglycansandcollagenproducedfromretroorbitalfibrohlasts(RFthatresultsinexophthalmos?Inpre—sentstudy.thestimulatingfactorstoRFinvitroforthepatientswithGOweinvestigated.MethodsIThepuri—fledIgGfrom10patientswithGravesophthalmopathy,7patientsw
5、ithGraves'disease?4patientswithtoxicmuhinodulargoiterand8normalpersonsandeytokineIFN-TwmincubatedwithhumanRFinvitroandthesyn—thesis0{hyaluronieacid(HA)andtype1VcollagenandRFproliferationweredetectced.R~ults:All0{theIgGfromthepatientswithGOandotherthyroiddiseaseandnormalpersonscouldsti
6、mulateRFwithincreaseoiHAsynthesis.buttherewerenosignificantdifferenceamongthem?IFN一couldincreasethesynthesisofHAinthehumanRF,whereasinhibitedthesynthesiso{DnpeIVcollagen.Conclusion{NostimulatingantibodiesspecificallydirectedtoRFinthepatientswithGOwerefoundinthisstudy.Theef{ectso{IFN一o
7、nthesynthesisofHAproducedinRFmightplayo.13importantroleinpathogellesisinGO.KEYWORDSGraves'ophthalmopathylretroorbitalfibroblasts;inter{eron-V;hyaluronlcacidGraves眼病(G0)的病理特点是球后结缔组织内大量葡萄糖胺聚糖(Glyoosaminoglyoans,GAGs)和胶原蛋白沉积.亲水的粘多糖导致球后组织水肿.已知眶后成纤维细胞(Retroorbitalfibroblasts,RF)是产生GAG的源细胞.
8、本研究试图
