抗氧化活性测定方法

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1、抗氧化方法一、DeterminationofSuperoxideAnionScavengingAbility（超氧阴离子清除能力）1、determinedbyaCLmethodinthepyrogallol-luminolsystemonaBPCLUltra-weakluminescenceanalyzer。（焦性没食子酸-发光胺，荧光检测）2、步骤：10mLsample(不同浓度)+50mL焦棓酸(6.25*10-4mol/L)+0.94mLofamixturecontainingluminol(0.05mol/L)andcarbonatebuffer(pH10.2)（发

2、光胺用pH10.2碳酸盐缓冲液配成0.05mol/L）3、Hi-V,800;Kv,-1;thespectralrangeofCL（波长范围）180-800nm;温度：30°C.总时间：300S，每2S读数一次。4、对照：不加样品（样品用水代替）。空白不加焦棓酸（用来调零）。二、DeterminationofScavengingAbilityonHydroxideRadicals（羟基自由基清除能力）1、CuSO4-Phen-Vc-H2O2CLsystem（1mL体系）2、50mLofsamplesolution（样品液）+50mLofa1.0mmol/LCuSO4solut

3、ion（CuSO4溶液）+50mLofa1mmol/L1,10-phenanthrolinesolution（邻二氮菲溶液）+700mLofa0.05mol/Lboratebuffer(pH9.0)（硼酸缓冲液）+100mLofa1mmol/Lascorbatesolution+50mLofa0.15%H2O2solution3、总时间400S，间隔3S。Hi-V,800;Kv,-1;thespectralrangeofCL（波长范围）180-800nm;温度：30°C.4、阳性对照：不加样品（样品用水代替）。空白不加H2O2（用来调零）。三、Determinationof

4、ScavengingEffectonHydrogenPeroxide（过氧化氢清除能力）1、luminol-H2O2system2、Theluminescentreactionwasinitiatedbymanuallyadding1mLofasolutioncontaining0.15mol/Lhydrogenperoxideand0.1mol/Lluminolperliterofcarbonatebuffer(0.05mol/L,pH9.4).Lightemissionvstimewasrecordedfor3minat2sintervals.步骤：0.15mol/L的

5、过氧化氢+0.1mol/L发光胺（用pH9.4的碳酸缓冲液配），总体系1ml.3、BPCLUltra-weakluminescenceanalyzer，Hi-V,800;Kv,-1;thespectralrangeofCL（波长范围）180-800nm;温度：30°C.4、Thecontrolwasperformedinthesamemannerinthemixturewithoutthesamplesolution,andthebackgroundwasdetectedwithoutH2O2addition。三、DeterminationofPreventingDNADa

6、mageEffect（DNA损伤）1、CuSO4-Phen-Vc-H2O2-DNACLsystem.2、步骤：Copperand1,10-phenanthrolinewerepremixedin0.1mol/LNaOAc/HOAc(pH5.5)buffer。3mg/mLDNAwasincubatedwithaphen-Cusolution.步骤：800mLofphen-Cu/DNAsolution+100mLof4.2*10-3mol/Lascorbate+200mLof6%H2O2+wereaddedwithoutintervaltoa100mLsamplesolutio

7、ntogiveafinalvolumeof1.2mL.（总体系1.2mL）3、ThekineticcurveofCLproducedinthephen/Cu/H2O2/ascorbatesystemwasimmediatelyrecorded.(Thecontrolwasperformedinthesamemannerinthemixturewithoutthesamplesolution,andthebackgroundwasdetectedwithoutH2O2addition.)4、1.0mmol/LCuSO