坐骨神经慢性压迫损伤性疼痛及脊髓背角p物质关系探究

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1、坐骨神经慢性压迫损伤性疼痛及脊髓背角P物质关系探究【摘要】目的观察大鼠坐骨神经慢性压迫性损伤(CCI)后脊髓背角P物质表达的变化，探讨P物质在疼痛发生机制中的作用。方法SD雄性大鼠60只,随机分为:A组：CCI组(30只)；B组：对照组(30只)。术前及术后3、7、14、28d分别测定大鼠热痛阈值、机械痛阈值和行为学评分。术后3、7、14、28d每组取4只，麻醉后用4%多聚甲醛灌注固定，取L��4-6�段脊髓，以备免疫组化，测定SP的变化。结果所有CCI动物从术后第3天起,出现明显的疼痛行为学改变和热痛阈值、机械痛阈值的降低，与对照组比较差异

2、有统计学意义(P<0．05或P<0．01)。免疫组织化学结果表明，A组术后术侧明显高于B组(P<0．05或P<0．01)；A组术侧明显高于健侧(P<0．05或P<0．01)；而B组仅在第4天术侧高于健侧(P<0．05)。结论慢性坐骨神经损伤后，脊髓背角SP的表达增加，而且表达增加与CCI大鼠的痛觉过敏、行为变化在时相上基本一致，说明CCI大鼠痛觉过敏与脊髓背角SP的表达增加有关。�【关键词】神经病理性疼痛；脊髓背角；P物质��TheincreaseofsubstancePexpressionin11dorsalhornofspinalcord

3、inratswithchronicconstrictionnerveinjuryZHANGRui,WANGQing-xiu,GUOHui-qing，etal.DepartmentofAnesthesiology,affiliatedDongfengHospitalofShiyanYunYangMedicalCollege,Hubei，442008,China�【Abstract】ObjectiveToobservetheexpressivevarietyofsubstancePinthedorsalhornofspinalcordonneur

4、opathicpainbychronicconstrictioninjury(CCI).MethodsSixtymaleSprague-Dawleyratswererandomlydividedintotwogroups:CCIgroup,controlgroup.Recordtheresponsivenesstomechanicalandthermalstimulibeforeandaftersciaticnerveconstrictioninjury.Immuno-histochemistrytechniquewasusedtomeasu

5、retheexpressionofsubstancePinthedorsalhornofspinalcordat3th,7th,14th,28thdaysfollowingsciaticnerveconstrictioninjury.ResultsTherats’responsivenesstomechanicalandthermalstimuliandpainbehaviorweresignificantlyincreasedonpostoperativedaysaftersciaticnerve11ligationcomparedwith

6、preoperative(P<0．05).TherelativeexpressionlevelofsubstancePwasobviouslyincreasedat3dayinthedorsalhornofspinalcordaftersciaticnerveconstrictioninjurycomparedwithpreoperative(P<0．05).IngroupAincreaseinsubstancePexpressioninthedorsalhornofspinalcordwasobviouslyincreasedcompare

7、dwithgroupB(P<0．05).ConclusionsTheCCIrats’sensitivitytothermalandmechanicalstimuliwassignificantlyincreasedfollowingsciaticnerveconstrictioninjury.ThiscouldberelatedtotheincreaseofsubstancePexpressioninthedorsalhornofspinalcord.�【Keywords】Neuropathicpain；Dorsalhornofspinalc

8、ord；SubstanceP�疼痛性疾患是困扰人类健康的严重问题，其中慢性疼痛是常见的病理痛之一,在慢性疼痛中，神经源性痛(neuropathicpain)的治疗至