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1、T细胞激活剂对Jurkat细胞重组激活基因表达的影响第四军医大学(JFourthMilMedUniv)20;!l)tIp;/j璺!::!?研究原着?1253文章编号:1000-2790(2006)14-1253-03T细胞激活剂对Jurkat细胞重组激活基因表达的影响邹红云,马骊,姚新生,温茜,王小宁(.南方医科大学生物技术学院分子免疫学研究所,广东广州510515,华南理工大学生物科学与工程学院,广东广州510641)EffectsofTcellactivatorsonmRNAexpressionofrecombination?activatin
2、ggenesinJurkatcellsZOUHong-Yun,MA厶,YAOXin-Sheng,WENQian,WANGXiao-N/ng2InstituteofMolecularImmunology,SchoolofBiotechnology,SouthemMedicalUniversity,Guangzhou510515,China,SchoolofBioscienceandBioengineering,SouthChinaUniversityofTechnology,Guangzhou510641,China【Abstract】AIM:Toi
3、nvestigatetheeffectsofTcellactivatorsonthemRNAexpressionofrecombination-activatinggenes(RAGs)inJurkatcells.METHoDS:AfterJurkatcellsweretreatedtllPHA(20ms/L),anti—CD3mAb(1:100),PMA(40g/L)+A23187(0.5p~lol/L)for6and12hrespectively,RAG-1andRAG-2mRNAweremeasuredbyRT-PCR.Semi—quanti
4、tativeanalysiswasusedtoevaluateRAG—andRAG-2mRNAlevelsindifferentgroups.RESULTS:Comparedwiththecontrolgroups,thelevelsofRAG—JmRNAweresignificantlylowerindifferenttreatedgroups(P<0.05),andRAG-mRNAlevelswereassociatedwithtreatmenttime.G-2mRNAlevelsinPHAtreatedgroupsweresignifi
5、cantlylowerthanthoseinthecontrolgroups(P<0.05).NoRAC.-2mRNAwasdetectedinPMA+A23187treatedgroups.However,RAG-2mRNAlevelwassignificantlyhigherinanti—CD3mAb(12h)treatedgroupthanthatincontrolgroups(P<0.05).CoNCLUSIoN:RAG-1andRAG-2mRNAwerecoexpressedinJurkatcellsandCOUldbereg
6、u—latedbyTcellactivators.TheresultsmaygiveacluethatJurkatcellsmaybeprovideanidealcelllinemodelforstudyingtheregu-lationofRAGsinperipheralTcells.【Keywords】Tcellactivators;Jurkatcells;recombination—activatinggenes;reversetranscriptasepolymerasechainreaction收稿日期:2005-09-23:接受日期:2
7、005-11-11基金项目:国家重点基础研究发展规划"973"项目资助(2001CB510008)通讯作者:王小宁.Tel:(020)87114240Email:xnwang@21cn.net;马骊.Tel:(020)6l648322Email:maryhmz@126.corn作者简介:邹红云.主治医师,博士生(导师王小宁).Tel:(020)61648322Email:yijiamin2000@163.com【摘要】目的:研究T细胞激活剂PHA,抗-CD3mAb,PMA+A23187对人T细胞白血病细胞株Jurkat重组激活基因(RAGs)mRN
8、A表达水平的影响.方法:采用RT-PCR法检测不同T细胞激活剂作用不同时间后Jurkat细胞中RAG-1,RAG-2mRN
