1型超广谱β-内酰胺酶的研究

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1、1型超广谱β-内酰胺酶的研究中国抗生紊杂志2006年12月第31卷第12期?729?文章编号:1001—8689(2006)12—0729—04大肠埃希菌产PER一1型超广谱p一内酰胺酶的研究胡龙华贾坤茹余方友.熊建球韩立中.倪语星.(1南昌大学第二附属医院,南昌330006;2温州医学院附属第一医院,温州325000;3上海交通大学附属瑞金医院,上海200025)摘要:目的检测大肠埃希菌产PER一1型超广谱p内酰胺酶的发生率及其耐药特点.方法采用双纸片增效确证试验进行超广谱内酰胺酶(ESBLs)的表型检测,对表型阳性菌株用聚合酶链反应(PCR)和PCR产物测序方法确定超广

2、谱内酰胺酶的基因型.结果167株大肠埃希菌中有5O株产ESBLs,占29.9%(50/167);6株产PER一1型ESBLs,占3.6%(6/167).6株产PER-1型ESBLs大肠埃希菌均对头孢他啶和头孢噻肟耐药.5株对头孢西丁耐药,4株对阿米卡星敏感.全部菌株对亚胺培南敏感.结论大肠埃希菌中检测到产PER一1型超广谱p.内酰胺酶,且其耐药和多重耐药性严重,应引起重视.关键词:大肠埃希菌}PER-1l内酰胺酶;耐药基因型中圈分类号:R378.21文献标识码:AStudyonPER一1typeextended—spectrumbyEscherichiacolip—iact

3、amaseproducedHuLong—hua.JiaKun—ru,YuFang—you.,XiongJian—qiu,HanLi—?zhongandNiYu?-Xing(1TheSecondAffiliatedHospitalofNanchangUniversity.Nanehang330006}2TheFirstAffiliatedHospitalofWenzhouMedicalCollege.Wenzhou325000;3RujinHospitalofShanghaiJiaotongUniversity,Shanghai200025)ABSTRACTObjective

4、ToinvestigatetheprevalenceofPER一1typeextendedspectrum~-lactamases(ESBIS)anditsantimicrobial—resistanceprofileinEscherichiacolf.MethodsOnehundredandsixty—sevenstrainsofEscherichiacoliweretested.AUofthemwereisolatedfromallkindsofclinicalspecimensfromfourhospitalsofNanchangcityfromAugust2002t

5、oJune2003.Antimicrobialsusceptibilitytests(AST)weredeterminedbyKirby—Bauerdiskdiffusiontest.Double—disktestandthree—dimensionextractiontestwereusedtodetermineESBIS.blaPER.1geneswereamplifiedbyPCR.TheproductsofPCRweresequencedtoidentifythe~-lactamasegenotype.ResultsThepositiveratesofESBISin

6、Escherichiacoliwere29.9%(50/167).SixisolatescarriedblaP阻lgene.TheblaP限lgenepositiveratesofEscherichiacoliis3.6(6/167).Among6isolatesearringblaPER.1,allwereresistanttocefotaximeandceftaxidime,2resistanttoamikacin,5resistanttocefoxitinandallsensitivetoimipenem.ConclusionPER一1口一lactamasesandt

7、heirencodinggenesaredetectedinEscherichiacoli.TheisolatesharbouringblaPERlareresistanttomanyantibiotics.KEYWORDSEscherichiacoli;PER一1;口一lactamases;Resistancegenotype收稿日期:2006—04—18基金项目.江西省卫生厅科研基金资助项目(20043046).作者简介:胡龙华.男.生于1963年,学士.生管技师.生要从事l临床微生物检验及其耐药机制的研究.?