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1、密固达治疗绝经后骨质疏松模型大鼠观察[摘要]目的:探讨密固达对去势后绝经骨质疏松模型大鼠的治疗作用。方法:建立雌性SD大鼠去势后绝经骨质疏松模型16只,随机分为A、B两组,每组8只,A组大鼠给予密固达治疗,B组大鼠去势手术后未行治疗。另选8只正常雌性大鼠作为空白对照组(C组)。喂养12周后测定大鼠骨密度,同时检测血清中骨钙素、骨保护素和骨保护素配体的水平。结果:与B组比较,A组大鼠的骨密度显著增加(P<0.01),血清中骨钙素含量和骨保护素配体/骨保护素值显著降低(P<0.01)。结论:密固达对去势大鼠的骨质疏松有良好的治疗作用,其作用可能与降低血清中骨钙素含量和骨保护素配体/骨保
2、护素值有关。�[关键词]密固达;骨质疏松;大鼠�[中图分类号]R681.1[文献标识码]A[文章编号]1671-7562(2010)04-0406-02�doi:10.3969/j.issn.1671-7562.2010.04.029��TreatmentwithAclastainpostmenopausalosteoporosisrats��LIWei-hong�1,LIYu-ming�2,CUIWei-ding�3,WUBin�2(1.DepartmentofGynecology,OfficialHospital7ofNanjingCity,Nanjing210018,Chin
3、a;2.DepartmentofOrthopaedics,OfficialHospitalofNanjingCity,Nanjing210018,China;3.DepartmentofOrthopaedics,theFirstAffiliatedHospital,NanjingMedicalUniversity,Nanjing210029,China)��[Abstract]Objective:TostudythetreatingeffectofAclastaonratpostmenopausalosteoporosis.Methods:Sixteenratsmodelsofpo
4、stmenopausalosteoporosiswereestablishedbyremovingtwoovariesoftherats.TheywererandomlydividedintogroupAandB,with8ratseach.TheratsingroupAwereadministratedwithAclasta,andtheratsingroupBwerenottreated.Eightnormalfemaleratswerechosenasthecontrols(groupC).Bonedensity,serumosteocalcin,osteprotegerin
5、andosteprotegerinligandwereassessedafter12weeks.Results:ThebonedensityingroupAwassignificantlyhigherthanthatingroupB(P<0.01),whiletheserumosteocalcinlevelandtheratioofosteprotegerinligand/osteprotegerinweresignificantlydecreased7ingroupA(P<0.01).Conclusion:ApplicationofAclastahasagoodtherapeut
6、iceffectonratpostmenopausalosteoporosis,whichmayberelatedtodecreasingserumosteocalcinlevelandtheratioofosteprotegerinligand/osteprotegerin.�[Keywords]Aclasta;postmenopausalosteoporosis;rats��绝经后骨质疏松为全球性健康问题之一。骨质疏松症的严重性仅次于心脑血管疾病,其发病率在我国老年人群中已有日渐攀升之势。创新药物密固达能全面提升骨密度,抑制破骨细胞的骨吸收,降低骨转换率,降低骨折风险。本研究探
7、讨密固达对去势后绝经骨质疏松模型大鼠的影响。�1材料与方法�1.1材料�将24只雌性SD大鼠(由南京医科大学实验动物中心提供)随机分成3组,每组8只。密固达(北京诺华公司),骨保护素、骨保护素配体的试剂盒(ADL公司),骨钙素试剂盒(Sigma公司)。�1.2方法�1.2.1模型建立718周雌性SD大鼠麻醉后背部双切口,剪断卵巢组织,置输卵管于腹腔,胃和肠管回位,缝合切口。建立去势绝经后骨质疏松大鼠模型16只,随机分为A、B两组,每组8只。A组大鼠给予密固达治疗,经皮
