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1、雷公藤多甙对急性移植物抗宿主病小鼠的作用论文于艳秋，张义侠，金玉楠，卢晓梅，王巍，张海鹏【摘要】本研究应用雷公藤多甙防治小鼠急性移植物抗宿主病。对受致死量照射的C57BL/6受鼠，注入供鼠BABC/c骨髓与脾淋巴细胞的混合液，并给予雷公藤多甙、环孢素A、甲氨蝶呤处理。用免疫组织化学法、流式细胞术和ELISA法检测各组小鼠T淋巴细胞、黏附分子、细胞因子。结果表明：所有异基因对照组小鼠.freelRNA的表达（P0.05）；上调血清中IL-10的水平（P0.05），但对IL-4的作用不显著（P0.05）。结论：雷公藤多甙具有明显抗aGVHD作用并
2、同时保留抗白血病效应，其作用机制与调节促炎/抑炎细胞因子的分泌和黏附分子的表达，以及抑制T淋巴细胞作用有关。【关键词】雷公藤多甙；移植物抗宿主病；T淋巴细胞；黏附分子；细胞因子GlucosidorumTripterygiiTororum(GTT)isakindofChinesetraditionalmedicine(CTM),forthefirsttimeusedinChinaasanimmunoregulatoryagententofautoimmunologicaldiseasessuchasrheumatoidarthritis(RA)
3、andnephroticsyndrome.Ourpreviousexperimentshophocytes,inhibitthesecretionofIL-2andexpressionofIL-2R,doolecules(CD11a,CD18)onthesurfaceofTcells.Anditcanprolongthesurvivaltimeofallogeneictransplantedskin.Itseffectonanti-rejectionisethylprednisolone.And,.freelshouchmorestronge
4、reffect,indicatingthatGTTcanmoderatelyorobviouslyinhibitthefunctionofTcellsandthesecretionofcytokines［1］.Furthermore,GTTmayhaveanti-inflammatoryandanti-tumoreffects.Graft-Versus-Hostdisease(GVHD),especiallyacuteGraft-Versus-Hostdisease(aGVHD)isamajorlethalplicationafterbone
5、marroedtoadoptGTTorbineGTTechanism.MaterialsandMethodsMiceDonormiceBALB/c(H-2d),female;syngeneictransplantationrecipientmiceBALB/c(H-2d),male;allogeneictransplantationrecipientmiceC57BL/6(H-2b)male.AllofthemyofSciences).InductionofaGVHDTherecipientsreceivedin,SIMENZ,Primus,
6、Germany)for6-8hoursbeforetransplantation.BonemarroaleBALB/c(H-2d)edium.BothBALB/candC57BL/6recipientsarrophocytesviacaudalvein.AssignmentandprocessAftertransplantation,therecipientslydividedintofollo.Theirfoodandicin(320mg/L)anderythromycin(250mg/L)inistratedl;inCsA+MTXgrou
7、pg/kgeverydayandMTX7mg/m2ontheday1,3,5and9;inGTTgroupg/kg;inGTT+CsAgroupg/kgandCsA2.5mg/kg.GTTg/mlbyTiceesptomsofaGVHD(iceofallo-BMTgrouphadthesignificantclinicalsymptomsofaGVHD,suchasiceofsyn-BMTgrouphadnot.TherecipientmiceofGTT,GTT+CsAandCsA+MTXgroupsshoore,inappetencecou
8、ldbeobservedinGTTandGTT+CsAgroup.Onday14afterBMT,thetissuesamplesofskin,lungandint
