资源描述：
《淋巴瘀滞对自体肾移植影响实验探究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、淋巴瘀滞对自体肾移植影响实验探究【摘要】目的探讨淋巴淤滞对自体肾移植的影响。方法选择家兔为实验对象，左侧肾脏用于自体肾移植模型和淋巴瘀滞模型的建立，右侧肾脏用于自体肾移植模型的建立，以做对照。分别于术后第4、7、14、28天处死实验兔。分别从淋巴淤滞肾和对照肾切取标本，用40g/L甲醛固定，石蜡包埋切片，HE染色。结果淋巴淤滞肾(左肾)周围组织明显水肿、粘连，随着术后时间的延长，水肿减轻但粘连加重。两侧肾的肾小球和肾小管未见明显变化。术后第4天时，淋巴淤滞肾的小叶间毛细淋巴管扩张明显，淋巴液淤积，间质呈多灶性淋巴细胞浸润并伴有水肿；术后第7天时，除上述变化外，肾间质淋巴细胞浸润
2、及水肿最明显；术后第14天时，小叶间毛细淋巴管萎缩，间质呈少量淋巴细胞浸润；术后第28天时，毛细淋巴管明显萎缩，并伴有肾小球毛细血管充血。结论淋巴淤滞对自体移植肾的组织学有一定影响，并可能是加重肾移植后急、慢性排斥反应发生的重要因素。�【关键词】淋巴淤滞；自体肾移植；组织学InfluenceoflymphstasisonautologousrenaltransplantationGUOJun,FANXue-pin,CHENNai-qing,�et9al�.AffiliatedHospitalofTaishanMedicalCollege,271000，China�【Abstra
3、ct】ObjectiveTosearchfortheinfluenceoflymphstasisonautologousrenaltransplantation.MethodsTotally16rabbitswereselected,leftreneswereusedtoestablishedthemodelsofautologousrenaltransplantationandmodelsoflymphstasis,rightrenesascomparedwiththeleftreneswereusedtoestablishedthemodelsofautologousren
4、altransplantationonly.Thespecimenswereobtainedfromthebilateralrenesafter4、7、14、28days,formalin-fixed,paraffin-embeddedsection,HEstaining.ResultsThesurroundingtissueoftheleftrenesedema,adhesion,withthetimeaftertheoperation,edemareduced,butadhesionincreased.Bilateralrenalglomeruliandtubulesnoo
5、bviouschanges.Thefourthdayaftertheoperation,lymphaticcapillariesbetweenlobuleofthelymphstasisreneswereobviously9expansion,lymphdeposition,mesenchymashowedmultifocallymphocyticinfiltrationwithedema.Theseventhdayaftertheoperation,inadditiontotheabovechanges,lymphocyteinfiltrationandedemaofthem
6、esenchymaweremostobviously.Thefourteenthdayaftertheoperation,lymphaticcapillariesbetweenlobulegetsatrophy,mesenchymashowedfewlymphocyticinfiltration.The28daysaftertheoperation,lymphaticcapillariesatrophysharply,accompaniedbyglomerulicapillarycongestion.ConclusionLymphstasisofautologousrenalt
7、ransplantationhadacertainimpacttothehistology,anditmaybetheimportantfactorstocausetheacuteandchronicrejectionafterrenaltransplantation.�【Keywords】Lymphstasis;Autologousrenaltransplantation;Histology近年来，中国开展临床肾移植已达459828例次。首次尸体肾移植1年肾存活率达80%以上，患者存活率达
