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1、益气活血复方对自发性高血压大鼠左室心肌形态结构的影响张纯,陈利国,戴勇,胡小勤,郑佩娥【关键词】益气活血复方;,,自发性高血压大鼠;,,,左室肥厚;,,形态结构 摘要:目的探讨益气活血复方对自发性高血压大鼠(spontaneouslyhypertensiverats,SHR)左室肥厚(leftventricularhypertrophy,LVH)及左室心肌形态结构的影响。方法雄性14周龄SHR50只,随机分为复方高剂量组(SHRY1)、复方中剂量组(SHRY2)、复方低剂量组(SHRY3)、模型对照组(SHRC)、硝苯地平组(SHRX)。监测尾动脉收缩压(systolicbloodpress
2、ure,SBP),每4周1次,8周后取材。测算心脏重量指数(heartassindex,LVMI);光镜、透射电镜观察左室心肌细胞形态结构的改变。结果(1)与SHRC组比较,灌胃4,8周后,各治疗组SBP显著降低(P<0.01);(2)与SHRC组比较,灌胃8周后,SHRY1、SHRY2及SHRX组HI均明显降低(P<0.05或P<0.01);(3)光镜观察发现:SHRC组心肌纤维增粗或萎缩、断裂或排列紊乱,部分心肌细胞核肥大或固缩;透射电镜观察发现:SHRC组可见部分心肌细胞核膜皱缩,核染色质凝聚、趋边,偶见核固缩,部分心肌细胞线粒体明显肿胀。与SHRC组比较,SHRY1组上述病变明显减轻;
3、SHRY2、SHRX组病变有所改善;SHRY3组病变改善不明显。结论益气活血复方、硝苯地平均能降低SBP、HI,减缓SHR左室肥厚形成、发展过程中左室组织结构的破坏。 关键词:益气活血复方;自发性高血压大鼠;左室肥厚;形态结构 EffectsofYiqihuoxueDecoctionontheMorphologicalStructureofMyocardialCellsinLeftVentricleofSpontaneouslyHypertensiveRats Abstract:ObjectiveToexploretheeffectsofYiqihuoxuedecoctiononleft
4、ventricularhypertrophyandthemorphologicalstructureofmyocardialcellsinleftventricleofspontaneouslyhypertensiverats(SHR).MethodsFiftyfourteenaleSHRlyinto5groups:SHRY1(treatedoderatedoseofYiqihuoxuedecoction),SHRY3(treatedeasuredbeforeandduringtreatmenteveryfourent,allratsassindex(LVMI)orphologicalands
5、tructuralchangesofmyocardialcellsinleftventricleofSHRsicroscopeandtransmissionelectronmicroscope(TEM).Results(1)After4or8ent,paredeachtreatmentgroupent,paredSHRY1,SHRY2andSHRXicroscopeusclefibersent,asehypertrophicnuclearorpyknosisofmyocardialcells.TheresultsunderTEMeleftventriculartissuesobtainedfr
6、omSHRCgroup,nuclearmembraneshrankandnuclearchromatincondensedandcongregatedundernuclearmembranelikespeckle,abuttingagainstthenuclearenvelope;andpyknosisemyocardialcellsarkedlysedisorganizedordisruptedmitochondrialcristaeentionedpathologicalchangesofSHRY1elioratedslightlyinSHRY2andSHRX;butthereprovem
7、entinSHRY3.ConclusionYiqihuoxuedecoctionandnifedipinecanreducetheSBP,HIandsloagesofleftventricleinSHRsduringtheprocessofLVH. Keyl药液含2g生药)。硝苯地平,10mg/片(广东华南制药厂生产,批号:040602)。 1.2动物及分组清洁级雄性14周龄SHR大鼠50只,