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1、中国水稻科学(ChinJRiceSci),２０１５,２９(４):３３５－３４２http://www．ricesci．cnDOI:１０．３９６９/ji．ssn．１００１Ｇ７２１６．２０１５．０４．００１３３５．水稻褐色颖壳新等位基因OsFBX３１０bh６的克隆鉴定徐霞张晓波施勇烽王惠梅黄奇娜奉保华李小红郭丹吴建利∗(中国水稻研究所水稻生物学国家重点实验室/国家水稻改良中心,杭州３１０００６;∗通讯联系人,EＧmail:beishangd＠１６３．com)bh６,ANewAlleleforBrownHullinRice
2、IdentificationofOsFBX３１０XUXia,ZHANGXiaoＧbo,SHIYongＧfeng,WANGHuiＧmei,HUANGQiＧna,FENGBaoＧhua,LIXiaoＧhong,GUODan,WUJianＧli∗(StateKeyLaboratoryofRiceBiology/ChineseNationalCenterforRiceImprovement,ChinaNationalRiceResearchInstitute,Hangzhou３１０００６,China;∗Correspond
3、ingauthor,EＧmail:beishangd＠１６３c．om)bh６,anewalleleforbrownhullinrice．XUXia,ZHANGXiaobo,SHIYongfeng,etal．IdentificationofOsFBX３１０ChinJRiceSci,２０１５,２９(４):３３５Ｇ３４２．Abstract:Abrownhull６(bh６)mutantofricewasisolatedfromanEMSＧinducedIR６４mutantbank．Thebrownpigmentsstart
4、toaccumulateinthehullsafterheadingandreachamaximumlevelinmatureseeds．The１０００Ｇgrainweightandpaniclelengthofbh６significantlydecreasedwhileotheragronomictraitsincludingplantheight,numberofproductivepaniclesperplant,numberoffilledgrainsperpanicleandseedＧsettingrat
5、ewerelargelysimilartothoseofthewildＧtypeIR６４．Analysisofpigmentcontentsindicatedthatthelevelsoftotalflavonoidsandanthocyanininbh６weresignificantlyincreased．Thebrownhullphenotypewascontrolledbyasinglerecessivegeneonthelongarmofchromosome９．Sequencinganalysisindic
6、atedthatasinglebasesubstitution(GＧA)atposition１０１３wasdetectedinthecandidategene(LOC_Os０９g１２１５０)encodinganFＧboxdomaincontainingprotein．Functionalcomplementationusingbh６,isanewalleleofOsFBX３１０thewildＧtypeallelecouldrescuethemutation．Themutation,designatedasOsFBX
7、３１０bh６functioningasaninhibitorofbrownhull．IsolationofOsFBX３１０providesusefulexperimentalmaterialsandwouldfacilitatethestudiesonmechanismofflavonoidmetabolisminmonocotplants．Keywords:OryzasativaL．;brownhullmutant;molecularmarker;OsFBX３１０;flavonoidmetabolism徐霞,张晓
8、波,施勇烽,等．水稻褐色颖壳新等位基因OsFBX３１０bh６的克隆鉴定．中国水稻科学,２０１５,２９(４):３３５Ｇ３４２．摘要:通过EMS诱变获得遗传稳定的水稻褐色颖壳突变体(brownhull６,简称bh６).与野生型IR６４相比,在自然条件下突变体的颖壳颜色自抽穗期开始逐渐加深直至蜡熟期颖壳呈深褐色,除千粒重和穗长显著降低外,其他农艺性状(株高、每株有
