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《the development of an efficient plant regeneration system from callus derived from root segments of garlic (allium sativum l.)》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。
1、PlanningtosubmittoInVitroCell.Dev.Biol.Thedevelopmentofanefficientplantregenerationsystemfromcallusderivedfromrootsegmentsofgarlic(AlliumsativumL.)Si-JunZheng,BettyHenken,FransA.Krens&ChrisKik*PlantResearchInternational,WageningenUniversityandResearchCenter,P.O.Box16,6700AAWageninge
2、n,TheNetherlands*Correspondingauthor:C.Kik,Tel:31317477001;Fax:31317418094;E-mail:C.Kik@plant.wag-ur.nl19SummaryRootsegmentsfrominvitroplantletsoffourwidelycultivatedgarlic(AlliumsativumL.)cultivarsinEurope(Messidrome,MoradodeCuenca,MorasolandPrintanor)wereusedforcallusinductionandp
3、lantregeneration.Eighttreatmentsforcallusinductionwithdifferentauxinandcytokininlevelswerecomparedfortheireffectoncallusinductionandlaterplantregeneration.Therewerenostatisticallysignificantdifferencesamongcultivarsforthenumberofrootsegmentsthatinducedcallus.Theaveragecallusinductio
4、nfrequencyfromrootsegmentswas34.7%.Aftercallusinductionfortwomonths,calluslinesweretransferredtoregenerationmedium,i.e.MS30supplementedwith1mg/l(4.6mM)kinetin.Regeneratedplantswereobtainedviasomaticembryogenesisandorganogenesis.Calliderivedfromdifferentexperimentswerequiteuniformwit
5、hrespecttotheirregenerationpotential.Alsoitwasfoundthatourregenerationsystemwascultivarindependent.Highlystatisticallysignificantdifferenceswerefoundinthefrequencyofshootregenerationamongdifferentcallusinductiontreatments.Whencytokininwaspresentduringcallusinduction,latershootregene
6、rationwashigh,rangingfrom30.10%to47.60%.Alreadyasmallamountofcytokinin(0.1mg/l2ip:0.5mM)inthecallusinductionmediumhadaprofoundeffectonlaterregeneration.Allinall,anefficientcallusinductionandplantregenerationsystemcouldbedevelopedfromsegmentstakenalongtheentirelengthoftheroots.Thissy
7、stemcanbeusedforgarlictransformation.19Keywords:callusinduction,garlic,invitroplantlets,plantregeneration,rootsegmentsIntroductionGarlic(AlliumsatiumL.)isaveryimportantandwidelycultivatedcropthathasbeendomesticatedalreadyforalongtimeandwhichisknownforitsculinaryandmedicinaluse.Uptil
8、lnow,garlicbreeding
