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1、HindawiPublishingCorporationJournalofBiomedicineandBiotechnologyVolume2012,ArticleID295167,8pagesdoi:10.1155/2012/295167ResearchArticleConditionedMediumfromAdiposeTissue-DerivedMesenchymalStemCellsInducesCD4+FOXP3+CellsandIncreasesIL-10SecretionEkaterinaIvanova-Todorova,1IvanBochev,2
2、RumenDimitrov,3KalinaBelemezova,3MilenaMourdjeva,3StanimirKyurkchiev,2PlamenKinov,4IskraAltankova,1andDobroslavKyurkchiev11LaboratoryofClinicalImmunology,UniversityHospitalSt.IvanRilski,MedicalUniversityofSofia,15Acad.IvanGeshovStreet,1431Sofia,Bulgaria2InstituteofReproductiveHealth,Ob
3、/GynHospitalDr.Shterev,25-31HristoBlagoevStreet,1330Sofia,Bulgaria3LaboratoryofMolecularImmunology,InstituteofBiologyandImmunologyofReproduction,BulgarianAcademyofSciences,73TsarigradskoShosseStreet,1113Sofia,Bulgaria4DepartmentofOrthopedicsandTraumatology,UniversityHospitalTsaritsaYoa
4、nna,MedicalUniversityofSofia,8ByaloMoreStreet,1527Sofia,BulgariaCorrespondenceshouldbeaddressedtoDobroslavKyurkchiev,dsk666@gmail.comReceived12June2012;Accepted5October2012AcademicEditor:Ken-ichiIsobeCopyright©2012EkaterinaIvanova-Todorovaetal.Thisisanopenaccessarticledistributedundert
5、heCreativeCommonsAttributionLicense,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperlycited.Mesenchymalstemcells(MSCs)areanewandpromisingtoolfortherapyofautoimmunedisorders.Inrecentyearstheirpossibilitytotakepartinthemodulationofthei
6、mmuneresponseisdiscussed.TheexactmechanismsforimmunoregulationrealizedbyMSCsarenotclearyet,butinteractionswithotherimmunoregulatorycellsmaybeinvolvedinthisprocess.TheinvestigationoftheinfluenceofMSCsontheexpressionofFoxP3andcytokinesecretionbyThelpercellswastheaimofthisstudy.Thelperce
7、llswereisolatedfromPBMCsbymagneticseparationandMSCswereisolatedfromhumanadiposetissue,andCD4+TcellswereculturedwithconditionalmediumofMSCs.Themethodswhichwereusedincludeflowcytometry,ELISA,andHumanProteomeprofilerkits.TheresultsdemonstratedthatsecretoryfactorsinMSCsconditionalmediumlea
8、dtoincreased
