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1、最新Inducedpluripotentstem(iPS)cells诱导多能干细胞（iPS）细胞-药学医学精品资料Nuclearreprogrammingtoapluripotentstatebythreeapproaches ShinyaYamanaka&HelenM.Blau NATURE
2、Vol465
3、10June2010
4、doi:10.1038/nature09229ThefusionexperimentsbyTada,SuraniandcolleaguesclearlyshowedthatEScellsandembryonicgermcel
5、lscontainfactorsthatcaninducereprogrammingandpluripotencyinsomaticcells*.Before2006,theprevailingviewwasthatnuclearreprogrammingtoapluripotentstateisahighlycomplexprocessthatmightentailthecooperationofupto100factors**.*Tada,M.,Tada,T.,Lefebvre,L.,Barton,S.C.&Surani,M.A.Embryoni
6、cgermcellsinduceepigeneticreprogrammingofsomaticnucleusinhybridcells.EMBOJ.16,6510–6520(1997).**Nuclearreprogrammingtoapluripotentstatebythreeapproaches ShinyaYamanaka&HelenM.Blau NATURE
7、Vol465
8、10June2010
9、doi:10.1038/nature09229Gene-ExpressionProfilesofiPSCellsInductionofPlurip
10、otentStemCellsfromMouseEmbryonicandAdultFibroblastCulturesbyDefinedFactors KazutoshiTakahashiandShinyaYamanaka Cell126,663–676,August25,2006PluripotencyofiPSCellsDerivedfromMEFsInductionofPluripotentStemCellsfromMouseEmbryonicandAdultFibroblastCulturesbyDefinedFactors Kazutoshi
11、TakahashiandShinyaYamanaka Cell126,663–676,August25,2006theyaredifferentwithregardstogeneexpressionandDNAmethylationpatterns,andfailtoproduceadultchimaeras.Generationofgermline-competentinducedpluripotentstemcellsKeisukeOkita,TomokoIchisaka&ShinyaYamanakaVol448
12、19July2007
13、doi:1
14、0.1038/nature05934InductionofPluripotentStemCellsfromAdultHumanFibroblastsbyDefinedFactorsKazutoshiTakahashi,KojiTanabe,MariOhnuki,MegumiNarita,TomokoIchisaka,KiichiroTomoda,andShinyaYamanaka1,2ThegenerationofiPScellsfromadulthumandermalfibroblastswiththesamefourfactors:Oct3/4,
15、Sox2,Klf4,andc-Myc.HumaniPScellsweresimilartohumanembryonicstem(ES)cellsinmorphology,proliferation,surfaceantigens,geneexpression,epigeneticstatusofpluripotentcell-specificgenes,andtelomeraseactivity.Furthermore,thesecellscoulddifferentiateintocelltypesofthethreegermlayersinvit
16、roandinteratomas.ThesefindingsdemonstratethatiPScellsc
