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ID:56930485
大小:15.15 MB
页数:66页
时间:2020-07-21
《生物化学与分子生物学PPT图片素材课件.ppt》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、生物化学与分子生物学PPT素材可根据需要选择取舍Metabolismof5-fluorouracil(5-FU)5-FUCarac,Efudex5-Fluorouridine5-FUMP5-FdUMPUridinephosphorylase5-FluorodeoxyuridineThymidinephosphorylaseOrotatephosphoribosyltransferaseThymidinekinase5-FUTP5-FdUTPRNADNA5’-nucleotidase5’-nucle
2、otidaseUridinekinaseATPADPATPADPPRPPWesternBlotting1.ResolveproteinsamplesonSDS-PAGE6.IncubatetheblotwithchemiluminescentHRPsubstrateandexposetofilm2.ElectrophoreticallytransferfractionatedproteinsfromgelontoPVDFmembrane3.Blockthemembranewithneutralp
3、rotein(BSAormilkcasein)4.Incubatethemembranewithprimaryantibodyspecifictotargetprotein5.IncubatethemembranewithHRP-labeledsecondaryantibodyspecifictoprimaryantibodyCholesterolSterolToolkitPregnenolonProgesteronAldosteronCortisolCorticosteroneAndrosten
4、dioneTestosteronEstriol17-Estradiol1,25-DihydroxycholecalciferolCholicacidCovalentmodificationofproteinsPoly(ADPribosyl)Polymerase(PARP)Poly(ADPribosyl)polymerase(PARP)NADNicotinamidePoly(ADPribosyl)polymerase(PARP)Poly(ADPribosyl)chainelongationandb
5、ranchingNicotinamideNADNADDNApolymeraseDNApolymeraseDNApolymeraseOligosacharyltransferaseN-glycosylationpathwayinERMann-P-DolicholtransferasesFlippaseDolicholPDolicholPPDolicholPPDolicholPPDolicholPPGlcNAc-PtransferaseGlcNActransferaseDolicholPDolicho
6、lPPDolicholPPGDP-MannosyltransferasesGlc-P-DolicholtransferasesDolicholPDolichol-pyrophofphataseCytosolER40S60SUTPCTPTMPPyrimidineNucleotideInterconversionsUMPATPADPH2OUDPNucleosidemonophosphatekinaseATPADPNucleosidediphosphatekinaseATPADPCTPsynthase
7、RibonucleotidereductasedeoxyUDPNucleosidediphosphatephosphohydrolasedeoxyUMPThymidylatesynthaseN5,N10-MethyleneTHFDHFTTPNucleosidemonophosphatekinaseNucleosidediphosphatekinaseMyo-Inositol-3-phosphateIns(3)P1Biosynthesisandmetabolismofphosphoinosit
8、idesGlucose6-pgosphateIns(3)P1synthaseIns(3)P1phosphataseMyo-InositolInsPtdInssynthasePtdInsMyo-inositolkinasePtdIns-4-PPtdIns4-kinasePtdIns5-kinasePtdIns-4,5-P2PI3KPtdIns-3,4,5-P3PLCPLCPI3KPtdIns
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