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时间:2020-06-04
《项丛刺针法对缺血性脑卒中后遗症大鼠BDNF、NGF以及神经行为学的影响.pdf》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、上海针灸杂志2014年2月第33卷第2期文章编号:1005-0957(2014)02—0181—04·动物实验·项丛刺针法对缺血性脑卒中后遗症大鼠BDNF、NGF以及神经行为学的影响章显宝,汪瑛,王震,孔红兵,汪节,江六顺,郭晓利,肖伟(安徽中医学院附属针灸医院,合肥230061)【摘要】目的观察项丛刺针法对缺血性脑卒中后遗症大鼠海马区BDNf、NGF以及神经行为学的影响。方法8O只雄性青年清洁健康级Wistar大鼠随机分为假手术组、模型组、治疗组、西药组,每组2O只。除假手术组外,其他动物均采用线栓法阻断大脑中动脉制备大鼠脑缺
2、血模型。疗程结束后,观察大鼠神经行为评分的变化,采用免疫组织化学染色方法检测各组大鼠海马区BDNF、NGF的表达。结果与假手术组比较,模型组大鼠海马区BDNF、NGF的表达显著升高(P3、血性脑卒中后遗症大鼠脑组织有神经保护和修复作用,疗效优于西药,其作用机制可能涉及有效上调大鼠BDNF、NGF蛋白的表达。【关键词】针刺:项丛刺针法:中风后遗症:BDNF:NGF:神经行为学:大鼠:穴位,头颈部【中图分类号】R2—03【文献标志码】ADOI:10.13460/j.issn.1005—0957.2014.02.0181EffectofNapeClusterAcupunctureonBDNF.NGFandNeurObehaviOrsinRatswithPost-ischemicStrokeSequelaeZHANGXi4、an—bao,WANGHng,WANGZhen,KONGHong-bing,WANGe,JIANGLiushun,GUOXiao—li,XIA0Wei.AnhuiCollegeofTraditionalChineseMedicine,Hefei230061,China『Abstract10biectiveToinvestigatetheeffectofnaDeclusteracupunctureonhippocampalBDNFandNGFandneurobehaviorsinratswithpost.ischemicstrok5、esequelae.MethodEightycleanandhealthyyoungmaleWistarratswererandomlyallocatedtoshamoperation,model,treatmentandWesterndruggroups,20ratseach.Aratmodelofcerebralischemiawasmadebythreadocclusionofthemiddlecerebralarteryinallthegroupsexcepttheshamoperationgroup.Afterthee6、ndoftreatment,ratneurobehaviorswerescoredandhippocampalBDNFandNGFexpressionswereexaminedbyimmunohistochemicalstainingineverygroupofrats.ResultComparedwiththeshamoperationgroupofrats,hippocampalBDNFandNGFexpressionsincreasedsignificantlyinthemodelgroup(P<0.01).Compare7、dwiththemodelgroupofrats,hippocampalBDNFandNGFexpressionsincreasedsignificantlyinboththetreatmentandWestemdruggroups(P<0.01).ComparedwiththeWestemdruggroup,theefectwasmarkedinthetreatmentgroup<0.05).Comparedwiththeshamoperationgroupofrats,theneurobehaviorscoreincreas8、edsignificantlyinthemodelgroup<0.01).Comparedwiththemodelgroupofrats,theneurobehaviorscoredecreasedsignificantlyinboththetreatmenta
3、血性脑卒中后遗症大鼠脑组织有神经保护和修复作用,疗效优于西药,其作用机制可能涉及有效上调大鼠BDNF、NGF蛋白的表达。【关键词】针刺:项丛刺针法:中风后遗症:BDNF:NGF:神经行为学:大鼠:穴位,头颈部【中图分类号】R2—03【文献标志码】ADOI:10.13460/j.issn.1005—0957.2014.02.0181EffectofNapeClusterAcupunctureonBDNF.NGFandNeurObehaviOrsinRatswithPost-ischemicStrokeSequelaeZHANGXi
4、an—bao,WANGHng,WANGZhen,KONGHong-bing,WANGe,JIANGLiushun,GUOXiao—li,XIA0Wei.AnhuiCollegeofTraditionalChineseMedicine,Hefei230061,China『Abstract10biectiveToinvestigatetheeffectofnaDeclusteracupunctureonhippocampalBDNFandNGFandneurobehaviorsinratswithpost.ischemicstrok
5、esequelae.MethodEightycleanandhealthyyoungmaleWistarratswererandomlyallocatedtoshamoperation,model,treatmentandWesterndruggroups,20ratseach.Aratmodelofcerebralischemiawasmadebythreadocclusionofthemiddlecerebralarteryinallthegroupsexcepttheshamoperationgroup.Afterthee
6、ndoftreatment,ratneurobehaviorswerescoredandhippocampalBDNFandNGFexpressionswereexaminedbyimmunohistochemicalstainingineverygroupofrats.ResultComparedwiththeshamoperationgroupofrats,hippocampalBDNFandNGFexpressionsincreasedsignificantlyinthemodelgroup(P<0.01).Compare
7、dwiththemodelgroupofrats,hippocampalBDNFandNGFexpressionsincreasedsignificantlyinboththetreatmentandWestemdruggroups(P<0.01).ComparedwiththeWestemdruggroup,theefectwasmarkedinthetreatmentgroup<0.05).Comparedwiththeshamoperationgroupofrats,theneurobehaviorscoreincreas
8、edsignificantlyinthemodelgroup<0.01).Comparedwiththemodelgroupofrats,theneurobehaviorscoredecreasedsignificantlyinboththetreatmenta
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