葡萄籽原花青素对睡眠呼吸暂停模式低氧大鼠海马区磷酸化p38MAPK和IL-1β表达的影响.pdf

《葡萄籽原花青素对睡眠呼吸暂停模式低氧大鼠海马区磷酸化p38MAPK和IL-1β表达的影响.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、中风与神经疾病杂志2013年11月弟30巷弟11别文章编号：1003-2754(2013)11-0989-04葡萄籽原花青素对睡眠呼吸暂停模式低氧大鼠海马区磷酸化p38MAPK和IL一1I3表达的影响赵雅宁，刘文倩，王红阳，窦娜，郭霞，景丽伟摘要：目的探讨葡萄籽原花青素对睡眠呼吸暂停模式低氧大鼠海马区磷酸化丝裂原活化蛋白激酶(p38MAPK)和白介素一1B(IL一113)表达的影响。方法80只雄性sD大鼠随机分成对照组、模型组及高、低剂量萄籽原花青素组。对照组暴露于空气中模型组暴露于低氧条件下(50ml／L，暴露时间每天8h，持续时间2、6w)。电镜观

2、察海马区神经细胞超微结构，免疫印迹检测磷酸化p38MAPK和IL一1B的表达变化，水迷宫测试动物学习记忆功能。结果与对照组比较，模型组神经细胞结构损伤、磷酸化p38MAPKIL一113的表达升高，水迷宫检测动物逃避潜伏期时间延长、穿台次数减少(P<0．05)；与模型组比较，葡萄籽原花青素能减轻神经细胞超微结构的损伤，降低磷酸化p38MAPK和IL一1B表达，水迷宫检测动物逃避潜伏期时间缩短、穿台次数增多(P<0．05)；上述变化在高剂量萄籽原花青素组最为显著(P<0．05)。结论葡萄籽原花青素能改善睡眠呼吸暂停模式低氧大鼠的学习记忆功能，与抑制海马区磷

3、酸化p38MAPK和IL．1B表达有关。关键词：阻塞性睡眠呼吸暂停；有丝分裂原活化蛋白激酶；白细胞介素；学习记忆中图分类号：R743文献标识码：AEfectofGrapeseedproanthocyanidinonphosphorylatedp38MAPKandIL-113inhippocampusinaratmodelofobstructivesleepapneahypoxiaZHAOYa—ning，LIUWen—qian，WANGhong-yang，eta1．(RehabilitationCollegeofHe—beiUnitedI西e，Tangs

4、han063000，China)Abstract：ObjectiveToinvestigatetheeffectsofGrapeseedproanthoeyanidinonexpressionofphosphorylatedp38MAPKandIL一1Binhippoeampusinaratmodelofobstructivesleepapneahypoxia．MethodWerandomlydivided80MaleSDratsintofourgroups：controlgroup，modelgroup，lowdoseofGrapeseedproan

5、thocyanidintreatmentgroupandhighdoseofGrapeseedproanthocyanidintreatmentgroup．Ratsincontrolgroupwereexposedinair，themodelgroupweresuf-feredfromintermittenthypoxiaconditions(50ml／L，8-hour—intermittenthypoxiaeveryday，andthedurationofexperimentwas2and6weeks，respectively)．Electronmi

6、croscopewasusedtoobservedneuronpathologyinhippocampalregion；West-erR-Blottingwasusedtoobservedtheexpressionsofphosphorylatedp38MAPKandinterleukinlBproteins．Morriswatermaze(MWM)wasusedtoassessmemory—learningfunction．ResultsComparedwiththecontrolgroup，neuronalpathologyinjurywassig

7、nificantlyinjured；Theexpressionsofphosphorylatedp38MAPKandIL一18proteinssignificantlyincreased(P<0．05)：MWMtestshowedthattheescapinglatencyprolongedandthefrequencyofcrossingtheplatformdecreased．Com—paredwithmodelgroup，phosphorylatedp38MAPKandIL一1BleveldecreasedinGrapeseedproanthoc

8、yanidin(P<0．05)andtheescapinglatencyshortedandt