节瓜、冬瓜重要性状多样性及亲缘关系的SRAP分析.pdf

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1、植物遗传资源学报2014，15(5)：1150．1155JoumalofP1antGeneticResourcesDOI：10．13430／j．cnki．jpgr．2014．05．034节瓜、冬瓜重要性状多样性及亲缘关系的SRAP分析乔燕春，林锦英，谢伟平，谢丽芳，李莲芳(广州市农业科学研究院，广州510308；广州市果树研究所，广州510405)摘要：以冬瓜、节瓜38份种质资源为材料，主要对萌芽率、单果重、瓜长、瓜宽、瓜皮色、瓜型、星点多少、果肉厚和果肉色共计9个性状的表型性状进行了研究，供试

2、材料9个性状的表型变异系数在9．12％～123．13％之间。尤以单果重的变异系数最大；遗传多样性指数(H)在0．122—1．859之间。SRAP结果显示：利用筛选出的2O对引物对38份节瓜、冬瓜材料进行扩增，共扩增出多态性条带323条，遗传相似系数在0．95—0．99之间，节瓜和冬瓜的遗传背景非常窄。冬瓜和黄毛型节瓜亲缘关系近于普通类型节瓜。本研究还解决了部分材料同名异物的问题，可为冬瓜和节瓜育种提供有价值的参考。关键词：冬瓜；节瓜；表型多样性；SRAP；遗传多样性GeneticRelation

3、shipofChieh—-quaandWax·-gourdontheMorphologicalPhenotypesandSRAPMarkersQIAOYah—chun，LINJin-ying，XIEWei—ping，XIELi-fang，LILian-fang(InstituteofVegetableofGuangzhouAcademyofAgriculturalSciences，Guangzhou510308；GuangzhouFruitResearchInstitute，Guangzhou5

4、10405)Abstract：Inthisstudy，geneticrelationshipof38representativegermplasmsofthechieh—quaandwax—gourdwasstudiedbasedontheinvestigationof9morphologicalcharactersandSRAPmarkers．Atotalof9characterswerestud—ied，includingthegerminationrate，singlefruitweigh

5、t，melonlength，width，skincolor，shape，starsnumber，andmelonfleshcolor．Thevariationcoeficientofthese9biologicalcharactersrangedfrom9．12％to123．13％，amongwhichthecoefficientofsinglefruitweightwasthemaximum，andtheirgeneticdiversityindex(H’)rangedfrom0．122to1

6、．859．TheSRAPmarkershowedthattotally323polymorphicfragmentsinthetested38chieh-quaandwax—-gourdmaterialsweregeneratedby20pairsofSRAPprimers．Themoleculargeneticsimilaritycoeficientrangedfrom0．95to0．99．Thegeneticbackgroundsgapbetweenchieh—quaandwax—gourd

7、wasverynarrow．Thegeneticrelationshipresultsshowedthatbothyellow—fuzzchieh-quaandwax—gourdwereclosetothetypeofcommonchieh-qua．Therefore，thechieh—quashouldnotbeconsideredasavariantofwax-gourd，commonchieh—qua，yellow-fuzzchieh—qua，andblack—fuzzchieh—quac

8、ouldbeconsideredaswax—gourdlocalvariety．Thisstudyalsoaddressedsomematerialhomonymproblems．Inaword，thisstudycouldprovidethevaluablereferenceforthechieh—quaandWax-gourdbreeding．Keywords：wax—gourd；chieh—qua；phenotypicdiversity；SRAP；geneticdiversity节瓜和冬瓜