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时间:2020-05-07
《乙型肝炎患者外周血单个核细胞中共价闭合环状DNA的检测及其临床意义-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、·214·中华传染病杂志2014年4月第32卷第4期ChinJInfectDis,April201.,!临床论著乙型肝炎患者外周血单个核细胞中共价闭合环状DNA的检测及其临床意义朱圣涛谢琴秀张亚飞江晓平胡向阳柯晶李旭【摘要】目的分析HBV感染者PBMC中HBVcccDNA与血清HBVDNA、HBsAg、HBeAg、肝组织学的相关性,并探讨其临床意义。方法选取108例HBV感染者,密度梯度离心法提取PBMC,实时荧光定量PCR检测PBMC中HBVcccDNA和血清HBVDNA,化学发光定量检测血清中HBsAg和HBeAg,其中59例慢性HBV感染者
2、行肝活组织检查。计数资料比较采用检验,计量资料比较采用相关性分析,计量资料不符合正态分布的采用非参数检验。结果108例HBV感染者PBMC中HBVcccDNA阳性59例,占54.6,其中15例肝功能衰竭患者HBVcccDNA阳性11例,明显高于8例急性乙型肝炎中2例阳性(一4.960,P<0.05)。根据患者血清HBVDNA载量分为>5lg、3~5lg和<3lg拷贝/mL组,其PBMC中HBVcccDNA阳性率分别为76.1(51/67)、5/18和13.0(3/23),HBVDNA高载量组与中、低载量组比较,差异有统计学意义(。值分别为14.7
3、51、28.384,均P<0.05)。PBMC中HBVcccDNA与血清HBVDNA载量和HBsAg呈正相关(r值分别为0.554、0.497,均P<0.05)。PBMC中HBVcccDNA在肝组织学≥G2和(或)s2组检出率明显高于4、cularDNAinperipheralbloodmononuclearcellsofhepatitisBpatientsanditsclinicalsignificanceZhuShengtao,XieQinxiu,ZhangYafei,JiangXiaoping,HuXiangyang,KeJin,LiXu.DepartmentofInfectiousDiseases,FirstAffiliatedHospitalofAnhuiMedicalUniversity,Hefei230032,ChinaC0rrs0,zgauthor:LiXu,Em5、ail:aylixu@qq.corn[Abstract]ObjectiveToanalyzethecorrelationbetweencovalentlyclosedcircularDNA(cccDNA)intheperiphera1bloodmononuclearcells(PBMC)ofhepatitisBvirus(HBV)一infectedpatientsandserumHBVDNA,hepatitisBsurfaceantigen(HBsAg),hepatitisBeantigen(HBeAg)andliverhistologyofhe6、patitisBpatients,andtoexploretheclinicalsignificanceofHBVcccDNAdetectioninPBMC.MethodsOnehundredandeightpatientswithchronicHBVinfectionwereinvolvedinthisstudy.PBMCwereextractedusingdensitygradientcentrifugation.HBVcccDNAinPBMCandserumHBVDNAweredetectedbyreal—timefluorescenceq7、uantitativepolymerasechainreaction.HBsAgandHBeAgweredetectedbychemiluminescenceimmunoassay.Liverbiopsywasconductedin59outofthe108patients.Chi-squaretestwasusedtocomparethecategoricalvariables.Correlationanalysiswasusedtocomparequantitativevariables.Nonparametrictestwasusedtoc8、omparethenon—norma1distributionparameters.ResultsIntheoverallpopulat
4、cularDNAinperipheralbloodmononuclearcellsofhepatitisBpatientsanditsclinicalsignificanceZhuShengtao,XieQinxiu,ZhangYafei,JiangXiaoping,HuXiangyang,KeJin,LiXu.DepartmentofInfectiousDiseases,FirstAffiliatedHospitalofAnhuiMedicalUniversity,Hefei230032,ChinaC0rrs0,zgauthor:LiXu,Em
5、ail:aylixu@qq.corn[Abstract]ObjectiveToanalyzethecorrelationbetweencovalentlyclosedcircularDNA(cccDNA)intheperiphera1bloodmononuclearcells(PBMC)ofhepatitisBvirus(HBV)一infectedpatientsandserumHBVDNA,hepatitisBsurfaceantigen(HBsAg),hepatitisBeantigen(HBeAg)andliverhistologyofhe
6、patitisBpatients,andtoexploretheclinicalsignificanceofHBVcccDNAdetectioninPBMC.MethodsOnehundredandeightpatientswithchronicHBVinfectionwereinvolvedinthisstudy.PBMCwereextractedusingdensitygradientcentrifugation.HBVcccDNAinPBMCandserumHBVDNAweredetectedbyreal—timefluorescenceq
7、uantitativepolymerasechainreaction.HBsAgandHBeAgweredetectedbychemiluminescenceimmunoassay.Liverbiopsywasconductedin59outofthe108patients.Chi-squaretestwasusedtocomparethecategoricalvariables.Correlationanalysiswasusedtocomparequantitativevariables.Nonparametrictestwasusedtoc
8、omparethenon—norma1distributionparameters.ResultsIntheoverallpopulat
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