GLPP对Alzheimer样大鼠空间记忆能力和海马超微结构的影响-论文.pdf

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1、河南职工医学院学报Vo1．25No．6Dec．2013JournalofHenanMedicalCollegeforStafandWorkers·663·基础医学GLPP对Alzheimer样大鼠空间记忆能力和海马超微结构的影响木李宜培，王黎△(河南医学高等专科学校病理生理学教研室，郑州451191)[摘要]目的观察GLPP对Alzheimer样大鼠记忆能力和海马超微结构的影响。方法雄性Wistar大鼠60只，随机分为4组，每组15只：正常对照组、模型组、Ns组、GLPP组(250mg／kg)；除正常对照组(正常昼夜节律)

2、外，其余各组连续光照(光照度400Lux，24h)，其间GLPP组igGLPP，每天1次；NS组培相同体积的生理盐水，每天1次；光照30d后Morris水迷宫法测试各组大鼠空间记忆能力，透射电镜观察海马线粒体和突触结构的改变。结果模型组大鼠寻台潜伏期明显延长，与正常对照组比较有显著性差异(P<0．05)；GLPP组寻台潜伏期明显缩短，与模型组比较有显著差异(P<0．05)；透射电镜结果显示：正常对照组、GLPP组大鼠海马线粒体神经轴突和神经突触基本正常；模型组、NS组大鼠海马线粒体肿胀、结构紊乱，膜结构破坏，嵴模糊、消失；

3、神经髓鞘内神经细丝稀少，神经突触缺失、减少，突触密度降低，突触间隙不清，突触小泡减少。结论GLPP可增强Alzheimer样大鼠空间记忆能力，减轻持续光照对大鼠海马超微结构的破坏。[关键词]GLPP；记忆能力；海马；线粒体；神经突触[中图分类号]R322．81[文献标识码]A[文章编号]1008—9276(2013)06—0663—04EffectofGLPPinAlzheimer·likeRatSpatialMemoryAbilityandItsUltramicrostructureLIYi．pei．WANGLi(Pat

4、hophysiolyDepartmentofHenanMedicalCollege，Zhengzhou，451191，China)[Abstract]ObjectiveToobservetheeffectofganodermalucidumpolysaccharidespeptide(GLPP)onspatialmemoryandthemitochondrion，neuralsynapseinhippocampusofAlzheimer-likerats．Method60maleWistarratsweredividedr

5、andomlyintofourgroups(15pergroup)：normalcontrols，modelgroup，NSgroupandGLPPgroup(250mg／kg)．Exceptfornormalcontrols(received12：12hrlight—darkcycle)，allothergroupswerereceivedcontinuousillumination(400luxilluminance)for30days．Duringtheilluminatingperiod，GLPPgroupwasr

6、eceivedGLPP(ig，onetimeperday)；NSgroupwasreceivednormalsalineofthesamevolumeastheGLPPgroup(ig，onetimeperday)．Afteranilluminat—ingperiodof30days，Morriswatermazetestwasusedtomeasurethespatialmemoryofrats．Transmis—sionelectronmicroscope(TEM)observedthechangesofmitocho

7、ndriaandneuralsynapsisinhippocam-pus．ResultComparedwithnormalcontrols，thespatialmemoryofmodelgroupwassignificantlyre-duced(P<0．05)．Comparedwithmodelcontrots，thespatialmemoryofGLPPgroupwassignificantlyenhanced(P<0．05)．Theresultsbythetransmissionelectronmicroscopesh

8、owedthatnormalcontrolgroupandGLPPgroupbearednormalmitochondriaandsynapse．However，forthemodelgroupandNSgroup，mitochondriawereswelling，structureinhippocam