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时间:2020-05-04
《大鼠自体原位肝移植后肺组织MD-2的表达及意义-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、塑丝壁瘦苤盍f皇主题l垫!至生!至旦箜垒鲞筮垒塑』堡igQ堡丛f曼!!!!!塑造∑!墅塑):望!!璺堡坚!婴!兰:!尘垒,№:垒265·论著·大鼠自体原位肝移植后肺组织MD.2的表达及意义高婉菱王艳玲张瑷兰黑子清池信锦【摘要】目的观察自体肝移植后肺组织病理变化及MD.2、TLR2/4基因和蛋白表达变化。方法选取健康SPF级sD大鼠30只,随机分为假手术对照组(s组,n=6)和移植肝脏再灌注后4、8、16、24小时组(M4、M8、M16和M24组,每组n=6)。观察肺组织病理损伤,Q.PCR法检测MD-2、TLR2/4基因表达.WesternBlot法分析MD.2、TLR2/4蛋白表达,EL
2、ISA法检测肺组织TNF.Ot和IL.6浓度。结果假手术组肺组织结构基本正常,移植组表现为肺间质明显出血、充血,中性粒细胞浸润显著增多,以肝脏再灌注后8小时最为明显。与S组相比,M4、M8、M16组肺组织MD.2、TLR2和TLR4基因与蛋白表达显著上调(P<0.05),在肝脏再灌注8小时时达到高峰。与S组比较,M8、M16和M24组肺组织匀浆的TNF.Ot和IL.6浓度显著升高,在肝脏再灌注8小时时达到高峰。结论大鼠自体原位肝移植术后肺组织MD.2、TLR2和TLR4基因与蛋白表达显著上调.TNF—a和IL.6浓度升高,可能对肝移植急性肺损伤起重要作用。【关键词】自体肝移植;急性肺损伤;
3、髓样分化蛋白.2MD-2genetranscriptionandexpressionlevelinlungtissuesinaratorthotopicautologusfivertransplantationmodelGAOWan-ling,WANGYan·ling,ZHANGAi-lan,HEIZi—qing,CHIXin—fin.DepanmentofAnesthesiology,the砚涮AffiliatedHospitalofSunYat—senUnivers毋,Guangzhou510630,ChinaCorrespondingauthor:CHIXin-jin,E—mail:
4、chixinjin@yeah.net【Abstract】ObjectiveToinvestigatethegenetranscriptionandexpressionlevelofmyeloiddifferentiationprotein一2(MD-2)inlungtissuesinaratorthotopicautologuslivertransplantationmodel.MethodsThirtyhealthySPFSprague—Dawleyratswererandomlydividedintofivegroups.Theratsweredividedintoshamgroup(
5、S,n=6),andM4,M8,M16,M24groupsrespectivelyaccordingtothedifferenthoumafterliverreperfusion(n=6,respectively).Lungpathologicalmorphologychangesafterliverreperfusionwereexamined.Q.PCRandwesternblottingmethodswereusedtodetectMD一2andTLR2/4levels.ThecontentsofTNF—OtandIL-6weregenestranscription,andprote
6、inexpressionmeasuredusingELISAkit.Resul缸Normalstructureofthetungtissuesandseldominflammatorycellsinfiltrationwerefoundinlungtissuesintheshamgroup,whilecollapseofthepulmonarystructurewithsevereinfiltrationofinflammatorycellsintotheintra-alveolarandinterstitialspacesandinterstitialinmodelwiththesham
7、edema,mostmarkedly8hafterliverreperfusiongroups。Comparedgroup,MD一2andTLR2/4andlevels.theconcentrationsofTNF—dandIL一6intissuesingeneproteinexpressionlungM4、M8、M16andM24groupssignificantlyincreasedandreachedthepeak
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