右美托咪定对严重烫伤大鼠心肌细胞凋亡的影响.pdf

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1、1202华麻阱：杂20I3／'1-l0Ji笫33卷10j9jChit·．j、Iiesthe㈨il，O㈨t，ber20I3，Vnl33，N(1_l()·麻醉并发症·右美托咪定对严重烫伤大鼠心肌细胞凋亡的影响王惠枢徐世元张良成郑艇钟毅蒋焕伟【摘要】目的价“荚托咪定埘蓖烫伤人鼠心肌胞捌I、一的影响方法健成‘l雄忡sI)人鼠18只，体重220～280g，采川随机数法，将分为3i(rl=6)：埘照((：)、烫f，_J乡I【(B)、烫伤+右荚托咪定30／kg组(f)21【)地火鼠30％体表面烫伤模型，敛伤按t)alklam1公式补液抗休兜治疗。I)绑伤即刻版

2、腔剁J天十E咪定30l*g／kg，13绀腹腔注射窬牛盐水r烫伤12h取屈心卒心肌组织，光学显做镜观察心J~JL21i织痫结，J、用FUNH法确定心J】J【细胞州r：指数，应用WcsIcrnbit，t法检洲心肌葡萄糖悯蛋h78(GRtr／8)、C／EBP源蚩(CHOt，)发结果jC比较，B组和1D组心肌细胞凋：指数外高、心肌GRP78及CHOP丧达卜凋(，<0．05)；B比较，D组心儿儿细胞捌亡指数降低、心』tJL(；RP78jCttOl丧达F洲(P<0．05)，FI心肌坝伤显，D组心肌病理伤叫显减轻。结论打火托眯r1f通过抑制，1霞烫伤夫鼠内质啦

3、激介的心肌细胞捌，发挥心肌保护作J1J『【关键词】托咪啶；烧伤；心肌；胞捌I、EffectofdexmedetomidineonapoptosisinmyocardialcellsinratswithseverescaldWANGIlui—shu．xt

4、Shi—yaall，ZI1A／~(；Liang—cheng，ZItENGTing，ZHONGYi，,IIANGtlaan—wei．Depattmer~to／’Ane,sthe,siolog~，AfiliatedUniontlospital(Fuji(1~rlMedical1／niversit)，F

5、uzhou350001，ChinaCorres1)otzdingauthor：XUShi-)aafl，DepartrttentArz~：sthesiolog)，ZhujiangHospitalo／、Southern．MedicalUrtiver—sit)．，Guangzhou510282，China．Emaif：XUshi)aan355@163．㈣m【Abstract】ObjectiveT¨evaluatethet“。cloldexm((1e

6、omidine(J『1ap【)Iltusisinmyocardial(-el1sitlratswiths

7、everescull】．MethodsEighteenhealthymaleSI)rague—I)awleyrats，weighing220—280g．weH、randomlyTIT—videdinlo3groups(n=6each)usingarandomnum1)eltable：controlgroup(groupC)，seaMgroul'(gloupB)antiseaM+dexmedetonfidine30l*g／kggroup(gn)LlIlD)．l'hiny[)erT：cn[otI}lItolalhoelySLIT['acewassha

8、vedandthenexposedto94℃watertor12s．Ratswereresuscita!edwithisotonicsalineaec(mtingtoParklamImulaim—mediatelyafterburn．ShamburnwasproducedinCgroup．Ingioup1)．theratsreceiw：~ditlraperiloneaIinjectionoldexmedetomidine30／,g／kgimmediatelyafterburn．ant]theequal'．／ohJ．nleofnorllla]sal

9、inewasinjett1iTIgioupB．Theleftventri(dewasremovedaL12hafterburnto()[)sciwethepathologi(：alchangesofnlyocaidialtissueswithlightmicroscopeandtodetecttheap(’pt()sisinmyocardial(：ells(TUNEIassay)andexpressic)n()fglucose_regulatedpro—tein78(GRP78)andC／EBP—hom()togousprotein(CIIOI)

10、(usingWesternlflnt)．rhan{qotosisindexwascah：ll—luted．Results(]ompare