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1、110生理学报ActaPhysiologicaSinica,April25,2006,58(2):110-115http://www.actaps.com.cn研究论文AcSDKP抑制体外培养条件下人骨髓间充质干细胞的增殖戴国，黄畅，李烨，皮亦华，汪保和*湖南师范大学生命科学学院，长沙410081摘要：N-乙酰基-丝氨酰-天冬氨酰-赖氨酰-脯氨酸(N-acetyl-seryl-aspartyl-lysyl-proline,AcSDKP)是一种具有生理调控活性的四肽因子，对造血干/祖细胞增殖具有抑制作用。本研究采用集落形成实验、甲基偶氮唑盐(MT
2、T)比色法、细胞分裂指数测定等方法,考察了AcSDKP对体外培养的人骨髓间充质干细胞(mesenchymalstemcell,MSC)增殖的影响。结果显-12-9示，在AcSDKP浓度为1×10mol/L~1×10mol/L的培养体系中，人骨髓MSC集落生成率和大小、活力细胞数和分裂指-11数均降低，最大效应浓度为1×10mol/L。以上实验结果表明，在体外培养条件下，一定浓度的AcSDKP对人骨髓MSC的增殖具有抑制作用。关键词：AcSDKP；增殖；抑制；间充质干细胞；骨髓中图分类号：Q253InhibitoryeffectsofAcSDKP
3、onproliferationofhumanbonemarrowmesenchymalstemcellsinvitroDAIGuo,HUANGChang,LIYe,PIYi-Hua,WANGBao-He*CollegeofLifeSciences,HunanNormalUniversity,Changsha410081,ChinaAbstract:ThetetrapeptideN-acetyl-seryl-aspartyl-lysyl-proline(AcSDKP),aphysiologicalregulatorofcellproliferat
4、ion,hasbeenprincipallyreportedasapotentinhibitoroftheproliferationofhaematopoieticstemcellsandprogenitors.ThepurposeofthisstudywastoinvestigatewhethertheAcSDKPmaydirectlyaffecttheproliferativepotentialofhumanbonemarrowmesenchymalstemcells(MSCs)invitro.WeaddedAcSDKPtothecultu
5、resofhumanbonemarrowmononuclearcellsandmeasuredthenumberandaverageareaofMSCcolonies.MTTcolorimetricassayandmitoticindexdeterminationwerefurtherusedtoexaminetheproliferativestateofthethirdpassageMSCsinsubcultureswithorwithouttheadditionofAcSDKP.Inaddition,weevaluatedwhetherAc
6、SDKPmaykillMSCsbythetrypanbluedyeexclusiontest.Theresultsshowedthatthecolonyformingcapacity,thenumberofviablecellsandthe-12-9mitoticindexwerereducedinhumanbonemarrowMSCsculturedin1×10mol/Lto1×10mol/LAcSDKP.Maximuminhibitory-11activityappearedin1×10mol/LofAcSDKP.Nodifferencei
7、npercentoflivingcellswasobservedbetweentheMSCsubcultureswithandwithouttheadditionofAcSDKP.Asaresult,AcSDKPwithinacertainrangeofconcentrationshasnegativelyregulatoryeffectsontheproliferationofhumanbonemarrowMSCsinvitro.Keywords:AcSDKP;proliferation;inhibition;mesenchymalstemc
8、ell;bonemarrowN-乙酰基-丝氨酰-天冬氨酰-赖氨酰-脯氨酸进入S期，使其停留在G期，而发挥对细胞增殖0[2,3](N-acetyl-se
