包芯结构骨支架修复兔桡骨缺损-论文.pdf

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1、中华实验外科杂志2014年3月第31卷第3期ChinJExpSurg，March2014，V．．·6l3··实验研究·包芯结构骨支架修复兔桡骨缺损王闯建张春霖吴学建朱旭李莹严旭【摘要】目的采用骨髓基质干细胞(BMSCs)复合包芯结构骨支架修复兔桡骨缺损。方法成骨诱导培养3周的BMSCs与包芯结构骨支架复合构建细胞支架复合体(A组)，BMSCs／聚乳酸羟基乙酸(PLGA)／[3磷酸三钙([3-TCP)复合体(B组)、空白缺损(C组)作为对照。采用两因素随机区组设计植入36只兔(72侧)桡骨1．5cm缺损部位，术后l2、24、48周取材，进行相关分析。结果大体观察、

2、影像学、组织学检测示术后12周，A组骨缺损两断端骨性相连，24周A组支架内部大量新生骨生成，髓腔部分再通，48周A组髓腔再通，骨缺损完全修复，Lane—Sandhu评分示A组(5、8、12分)骨缺损修复效果明显好于B组(1、4、6分)、C组(0、0、1分)(P<0．05，n：4)。结论BMSCs／包芯结构骨支架复合体可修复兔桡骨1．5cm缺损。【关键词】包芯结构骨支架；骨髓基质干细胞；大段骨缺损；骨组织工程Repairofrabbitsegmentalradialdefectbyusingbonemesenchymalstemcellsintothewrappe

3、d-corestructurecompositescafoldWangChuangfian，ZhangChunlin，WuXuejian，ZhuXu，LiYing，YanXu．DepartmentofOrthopaedics，theFirstAffiliatedHospitalofZhengzhouUniversity，Zheng—zhou450052，ChinaCorrespondingauthor：WangChuangfian，Email：wangchuang20051983@126．corn【Abstract】ObjectiveTorepairsegmen

4、talradialdefectbyusingbonemesenchyma!stemcells(BMSCs)culturedinvitrofor3weeksunderosteogenicmediumintoaporouswrapped—corestructureCOB—positescaffoldcomposedofpoly(1actic—co—glycolicacid)／[3一tricalciumph0sphate(PLGA／13一TCP)skeletonwrappedwithtypeIcollagen．Methods1．5cmsegmentalradialde

5、fectswerecreatedbilaterallyin36rab-bits，thenBMSCsincombinationwithwrapped—corestructurecompositescaffold(groupA)andBMSCsincombinationwithPLGA／[3一TCPskeleton(groupB)weretransplantedintotheuntreatedradialdefeetasblankcontrolgroup(groupC)．12，24and48weeksafterimplantation，12animalsweresa

6、crificedandtheconstructswereharvestedandanalyzed．ResultsIngroupA，synosteosisformedbetweenthetwoendsofthecritical—sizebonedefect12weekspostoperation：boneintegritywasalmostfinishedandbonemarrowstartedatrendofrecanalization24weekspostoperation；bonedefectrepairwascompletedandbonemarrowwa

7、srecanalized48weekslater．AscomparedwithgroupB(1，4，6points)，andgroupC(0，0，1point)，Lane—SandhuindicatedthatthenewboneformationingroupA(5，8，and12points)wassignifi—cantlyincreased(P<0．05，n=4)．ConclusionThecombinationofBMSCsandcore—sheathstructurecompositescaffoldexhibitsafavorableosteoge

8、nesis，biocom